Plasma free metanephrines in diagnostics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10295221" target="_blank" >RIV/00216208:11110/14:10295221 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf" target="_blank" >http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Plasma free metanephrines in diagnostics

Popis výsledku v původním jazyce

Quantitative determination of catecholamines and their O-methyl metabolites (metanephrines) plays an important role in the diagnosis of pheochromocytoma (PHEO) - adrenal medulla tumour. The most common symptom of this kind of tumour is hypertension (high blood pressure). PHEO synthesizes, stocks, metabolizes and mostly secretes catecholamines. For this reason it is possible to use significantly elevated concentrations of catecholamines and their metabolic products as diagnostic markers of this tumour. The determination of metanephrines, mainly normetanephrine (NMN), metanephrine (MN) and 3-methoxytyramine (3-MT), is preferred against the determination of catecholamines because tumour cells produce free metanephrines continuously and irrespective of the release of catecholamines. The project aims to develop a new kit for the determination of plasma metanephrines. Solid phase extraction (SPE) is used for the pre-treatment of plasma samples. The determination is performed by ion-pair ultra-high performance liquid chromatography with electrochemical detection (UHPLC-ED).

Název v anglickém jazyce

Plasma free metanephrines in diagnostics

Popis výsledku anglicky

Quantitative determination of catecholamines and their O-methyl metabolites (metanephrines) plays an important role in the diagnosis of pheochromocytoma (PHEO) - adrenal medulla tumour. The most common symptom of this kind of tumour is hypertension (high blood pressure). PHEO synthesizes, stocks, metabolizes and mostly secretes catecholamines. For this reason it is possible to use significantly elevated concentrations of catecholamines and their metabolic products as diagnostic markers of this tumour. The determination of metanephrines, mainly normetanephrine (NMN), metanephrine (MN) and 3-methoxytyramine (3-MT), is preferred against the determination of catecholamines because tumour cells produce free metanephrines continuously and irrespective of the release of catecholamines. The project aims to develop a new kit for the determination of plasma metanephrines. Solid phase extraction (SPE) is used for the pre-treatment of plasma samples. The determination is performed by ion-pair ultra-high performance liquid chromatography with electrochemical detection (UHPLC-ED).

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/FR-TI4%2F331" target="_blank" >FR-TI4/331: *Vývoj kitu pro stanovení plazmatických metanefrinů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

CECE 2014: 11th International Interdisciplinary Meeting on Bioanalysis

ISBN

978-80-904959-2-0

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

361-364

Název nakladatele

Institute of Analytical Chemistry AS CR

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

20. 10. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000354547400117