Live imaging reveals spatial separation of parental chromatin until the four-cell stage in Caenorhabditis elegans embryos

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F16%3A10325947" target="_blank" >RIV/00216208:11110/16:10325947 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1387/ijdb.150222cl" target="_blank" >http://dx.doi.org/10.1387/ijdb.150222cl</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1387/ijdb.150222cl" target="_blank" >10.1387/ijdb.150222cl</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Live imaging reveals spatial separation of parental chromatin until the four-cell stage in Caenorhabditis elegans embryos

Popis výsledku v původním jazyce

The parental genomes are initially spatially separated in each pronucleus after fertilization. Here we have used green-to-red photoconversion of Dendra2-H2B-labeled pronuclei to distinguish maternal and paternal chromatin domains and to track their spatial distribution in living Caenorhabditis elegans embryos starting shortly after fertilization. Intermingling of the parental chromatin did not occur until after the division of the AB and P1 blastomeres, at the 4-cell stage. Unexpectedly, we observed that the intermingling of chromatin did not take place during mitosis or during chromatin decondensation, but rather similar to 3-5 minutes into the cell cycle. Furthermore, unlike what has been observed in mammalian cells, the relative spatial positioning of chromatin domains remained largely unchanged during prometaphase in the early C. elegans embryo. Live imaging of photoconverted chromatin also allowed us to detect a reproducible 180 degrees rotation of the nuclei during cytokinesis of the one-cell embryo. Imaging of fluorescently-labeled P granules and polar bodies showed that the entire embryo rotates during the first cell division. To our knowledge, we report here the first live observation of the initial separation and subsequent mixing of parental chromatin domains during embryogenesis.

Název v anglickém jazyce

Live imaging reveals spatial separation of parental chromatin until the four-cell stage in Caenorhabditis elegans embryos

Popis výsledku anglicky

The parental genomes are initially spatially separated in each pronucleus after fertilization. Here we have used green-to-red photoconversion of Dendra2-H2B-labeled pronuclei to distinguish maternal and paternal chromatin domains and to track their spatial distribution in living Caenorhabditis elegans embryos starting shortly after fertilization. Intermingling of the parental chromatin did not occur until after the division of the AB and P1 blastomeres, at the 4-cell stage. Unexpectedly, we observed that the intermingling of chromatin did not take place during mitosis or during chromatin decondensation, but rather similar to 3-5 minutes into the cell cycle. Furthermore, unlike what has been observed in mammalian cells, the relative spatial positioning of chromatin domains remained largely unchanged during prometaphase in the early C. elegans embryo. Live imaging of photoconverted chromatin also allowed us to detect a reproducible 180 degrees rotation of the nuclei during cytokinesis of the one-cell embryo. Imaging of fluorescently-labeled P granules and polar bodies showed that the entire embryo rotates during the first cell division. To our knowledge, we report here the first live observation of the initial separation and subsequent mixing of parental chromatin domains during embryogenesis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Developmental Biology

ISSN

0214-6282

e-ISSN

—

Svazek periodika

60

Číslo periodika v rámci svazku

1-3

Stát vydavatele periodika

ES - Španělské království

Počet stran výsledku

8

Strana od-do

5-12

Kód UT WoS článku

000375173000002

EID výsledku v databázi Scopus

2-s2.0-84960463913