Molecular markers of systemic autoimmune disorders: the expression of MHC-located HSP70 genes is significantly associated with autoimmunity development

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10362018" target="_blank" >RIV/00216208:11110/17:10362018 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023001:_____/17:00060293 RIV/00023728:_____/17:N0000057

Výsledek na webu

<a href="http://www.clinexprheumatol.org/abstract.asp?a=10147" target="_blank" >http://www.clinexprheumatol.org/abstract.asp?a=10147</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular markers of systemic autoimmune disorders: the expression of MHC-located HSP70 genes is significantly associated with autoimmunity development

Popis výsledku v původním jazyce

Objective To analyse the expression regulation of two inducible HSP70 genes - HSPAIA and HSPAIB - located within the major histocompatibility complex (MHC) in patients with various systemic autoimmune diseases and to prove the reliability of MHC-located HSP70 genes as molecular markers reflecting the autoimmune process. Methods 94 adult patients with idiopathic inflammatory myopathy (IIM, n=31), systemic lupus erythematosus (SLE, n=31) or systemic sclerosis (SSc, n=32) and 37 healthy individuals were analysed. The mRNA expression level was determined using quantitative real-time PCR method. The expression of intracellular HSP70 was established by flow cytometry. the extracelhdar HSP70 protein was measured in plasma samples using a commercially available sandwich enzyme-linked inununosorbent assay (ELISA). Results The expression of HSPAIA gene was significantly up -regulated in patients with autoimmune diseases (SLE: p&lt;0.01; SSc: p&lt;0.01; IIM: p&lt;0.0001) compared to healthy controls. The expression of HSPAIB gene was increased only in patients with myosins (p&lt;0.05). Furthermore, the HSPAIB gene expression is associated with the HLA-DRB1*03 risk allele in patients with IIM. In addition, we have found a relation between HSPAIA gene expression regulation and the presence of disease specific autoantibodies in patients with SLE and myosins. The level of intracellular HSP70 was not increased; however, the level of extracelhdar HSP70 protein was increased in patients suffering from SSc and IIM as compared to controls. Conclusion The results suggest an involvement of the MHC-linked HSP70 genes in the pathology of studied autoimmune disorders. Therefore, the HSPAIA and HSPAIB genes might serve as an interesting candidate molecule for development of distinct types of autoimmunities.

Název v anglickém jazyce

Molecular markers of systemic autoimmune disorders: the expression of MHC-located HSP70 genes is significantly associated with autoimmunity development

Popis výsledku anglicky

Objective To analyse the expression regulation of two inducible HSP70 genes - HSPAIA and HSPAIB - located within the major histocompatibility complex (MHC) in patients with various systemic autoimmune diseases and to prove the reliability of MHC-located HSP70 genes as molecular markers reflecting the autoimmune process. Methods 94 adult patients with idiopathic inflammatory myopathy (IIM, n=31), systemic lupus erythematosus (SLE, n=31) or systemic sclerosis (SSc, n=32) and 37 healthy individuals were analysed. The mRNA expression level was determined using quantitative real-time PCR method. The expression of intracellular HSP70 was established by flow cytometry. the extracelhdar HSP70 protein was measured in plasma samples using a commercially available sandwich enzyme-linked inununosorbent assay (ELISA). Results The expression of HSPAIA gene was significantly up -regulated in patients with autoimmune diseases (SLE: p&lt;0.01; SSc: p&lt;0.01; IIM: p&lt;0.0001) compared to healthy controls. The expression of HSPAIB gene was increased only in patients with myosins (p&lt;0.05). Furthermore, the HSPAIB gene expression is associated with the HLA-DRB1*03 risk allele in patients with IIM. In addition, we have found a relation between HSPAIA gene expression regulation and the presence of disease specific autoantibodies in patients with SLE and myosins. The level of intracellular HSP70 was not increased; however, the level of extracelhdar HSP70 protein was increased in patients suffering from SSc and IIM as compared to controls. Conclusion The results suggest an involvement of the MHC-linked HSP70 genes in the pathology of studied autoimmune disorders. Therefore, the HSPAIA and HSPAIB genes might serve as an interesting candidate molecule for development of distinct types of autoimmunities.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30226 - Rheumatology

Projekt

<a href="/cs/project/NT13699" target="_blank" >NT13699: Regulace exprese HSP70 genů jako potenciální marker pro monitoring myositidy.</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinical and Experimental Rheumatology

ISSN

0392-856X

e-ISSN

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Svazek periodika

35

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

10

Strana od-do

33-42

Kód UT WoS článku

000394415500005

EID výsledku v databázi Scopus

2-s2.0-85013150728