Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F21%3A10441267" target="_blank" >RIV/00216208:11110/21:10441267 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/978-1-0716-1522-5_26" target="_blank" >https://doi.org/10.1007/978-1-0716-1522-5_26</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-1522-5_26" target="_blank" >10.1007/978-1-0716-1522-5_26</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels

Popis výsledku v původním jazyce

Neuronal voltage-gated calcium channels play an essential role for calcium entry into presynaptic endings responsible for the release of neurotransmitters. In turn, and in order to fine-tune synaptic activity, numerous neurotransmitters exert a potent negative feedback over the calcium signal provided by G-protein-coupled receptors that can be recognized by characteristic biophysical modifications of the calcium current. There are two main biophysical approaches to analyze direct G-protein regulation of voltage-gated calcium channels: the so-called &quot;double pulse&quot; method, which is indirectly assessed by the gain of current produced by a depolarizing prepulse potential, and the &quot;subtraction&quot; method that allows the analysis of G-protein regulation from the ionic currents induced by regular depolarizing pulses. The later method separates the ionic currents due to nonregulated channels from the ion currents that result from a progressive departure of G-proteins from regulated channels, thereby providing valuable information on the OFF kinetics of G-protein regulation. In this chapter, we introduce these &quot;double pulses&quot; and &quot;subtraction&quot; procedures for use primarily with single cells, and also discuss the limitations inherent to these two approaches.

Název v anglickém jazyce

Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels

Popis výsledku anglicky

Neuronal voltage-gated calcium channels play an essential role for calcium entry into presynaptic endings responsible for the release of neurotransmitters. In turn, and in order to fine-tune synaptic activity, numerous neurotransmitters exert a potent negative feedback over the calcium signal provided by G-protein-coupled receptors that can be recognized by characteristic biophysical modifications of the calcium current. There are two main biophysical approaches to analyze direct G-protein regulation of voltage-gated calcium channels: the so-called &quot;double pulse&quot; method, which is indirectly assessed by the gain of current produced by a depolarizing prepulse potential, and the &quot;subtraction&quot; method that allows the analysis of G-protein regulation from the ionic currents induced by regular depolarizing pulses. The later method separates the ionic currents due to nonregulated channels from the ion currents that result from a progressive departure of G-proteins from regulated channels, thereby providing valuable information on the OFF kinetics of G-protein regulation. In this chapter, we introduce these &quot;double pulses&quot; and &quot;subtraction&quot; procedures for use primarily with single cells, and also discuss the limitations inherent to these two approaches.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

30105 - Physiology (including cytology)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Receptor and Ion Channel Detection in the Brain

ISBN

978-1-07-161521-8

Počet stran výsledku

11

Strana od-do

429-439

Počet stran knihy

554

Název nakladatele

Humana

Místo vydání

New York

Kód UT WoS kapitoly

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