Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00459795" target="_blank" >RIV/61388963:_____/16:00459795 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-4939-3064-7_22" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-3064-7_22</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-3064-7_22" target="_blank" >10.1007/978-1-4939-3064-7_22</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels

Popis výsledku v původním jazyce

Neuronal voltage-gated calcium channels play an essential role for calcium entry into presynaptic endings responsible for the release of neurotransmitters. In turn, and in order to fine tune synaptic activity, numerous neurotransmitters exert a potent negative feedback over the calcium signal provided by G-protein-coupled receptors that can be recognized by characteristic biophysical modifications of the calcium current. There are two main biophysical approaches to analyze direct G-protein regulation of voltage-gated calcium channels: the so-called double-pulse method, which is indirectly assessed by the gain of current produced by a depolarizing prepulse potential, and the "subtraction" method that allows the analysis of G-protein regulation from the ionic currents induced by regular depolarizing pulses. The later method separates the ionic currents due to nonregulated channels from the ion currents that result from a progressive departure of G-proteins from regulated channels, thereby providing valuable information on the OFF kinetics of G-protein regulation. In this chapter, we introduce these "double pulses" and "subtraction" procedures for use primarily with single cells and also discuss the limitations inherent to these two approaches.

Název v anglickém jazyce

Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels

Popis výsledku anglicky

Neuronal voltage-gated calcium channels play an essential role for calcium entry into presynaptic endings responsible for the release of neurotransmitters. In turn, and in order to fine tune synaptic activity, numerous neurotransmitters exert a potent negative feedback over the calcium signal provided by G-protein-coupled receptors that can be recognized by characteristic biophysical modifications of the calcium current. There are two main biophysical approaches to analyze direct G-protein regulation of voltage-gated calcium channels: the so-called double-pulse method, which is indirectly assessed by the gain of current produced by a depolarizing prepulse potential, and the "subtraction" method that allows the analysis of G-protein regulation from the ionic currents induced by regular depolarizing pulses. The later method separates the ionic currents due to nonregulated channels from the ion currents that result from a progressive departure of G-proteins from regulated channels, thereby providing valuable information on the OFF kinetics of G-protein regulation. In this chapter, we introduce these "double pulses" and "subtraction" procedures for use primarily with single cells and also discuss the limitations inherent to these two approaches.

Druh

C - Kapitola v odborné knize

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Receptor and Ion Channel Detection in the Brain: Methods and Protocols

ISBN

978-1-4939-3063-0

Počet stran výsledku

12

Strana od-do

357-368

Počet stran knihy

475

Název nakladatele

Humana Press

Místo vydání

New York

Kód UT WoS kapitoly

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