Determination of midazolam in rabbit plasma by GC and LC following nasal and ocular administration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F13%3A43907514" target="_blank" >RIV/00216208:11120/13:43907514 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023001:_____/13:00058736

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jssc.201300401" target="_blank" >http://dx.doi.org/10.1002/jssc.201300401</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201300401" target="_blank" >10.1002/jssc.201300401</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of midazolam in rabbit plasma by GC and LC following nasal and ocular administration

Popis výsledku v původním jazyce

GC with nitrogen phosphorus detection and HPLC with UV detection were used to determine midazolam levels in rabbit plasma following ocular and nasal administration. For GC with nitrogen phosphorus detection, the analyte was extracted from the plasma using a three-step liquid?liquid extraction including extraction with an isopropanol/butyl chloride mixture in an alkaline solution, followed by extractions with 1 M HCl and finally with an alkaline solution of butyl chloride. The recovery of midazolam was very dependent on the sample alkalization time prior to the final extraction. The procedure increased the recovery of midazolam up to 99.6%. Improved sample preparation led to a significant increase in the sensitivity of the determination by GC with nitrogen phosphorus detection. The achieved detection limit was 0.34 ng/ml, which is ten times lower than that obtained using HPLC with UV detection. The small plasma volume was another advantage of the GC with nitrogen phosphorus detection me

Název v anglickém jazyce

Determination of midazolam in rabbit plasma by GC and LC following nasal and ocular administration

Popis výsledku anglicky

GC with nitrogen phosphorus detection and HPLC with UV detection were used to determine midazolam levels in rabbit plasma following ocular and nasal administration. For GC with nitrogen phosphorus detection, the analyte was extracted from the plasma using a three-step liquid?liquid extraction including extraction with an isopropanol/butyl chloride mixture in an alkaline solution, followed by extractions with 1 M HCl and finally with an alkaline solution of butyl chloride. The recovery of midazolam was very dependent on the sample alkalization time prior to the final extraction. The procedure increased the recovery of midazolam up to 99.6%. Improved sample preparation led to a significant increase in the sensitivity of the determination by GC with nitrogen phosphorus detection. The achieved detection limit was 0.34 ng/ml, which is ten times lower than that obtained using HPLC with UV detection. The small plasma volume was another advantage of the GC with nitrogen phosphorus detection me

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/NT11284" target="_blank" >NT11284: Netradiční způsoby aplikace anestetik v medicíně katastrof</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

20

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

3366-3371

Kód UT WoS článku

000325482500009

EID výsledku v databázi Scopus

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