Epithelial cell morphology and adhesion on diamond films deposited and chemically modified by plasma processes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F14%3A43908867" target="_blank" >RIV/00216208:11120/14:43908867 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1116/1.4890471" target="_blank" >http://dx.doi.org/10.1116/1.4890471</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1116/1.4890471" target="_blank" >10.1116/1.4890471</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Epithelial cell morphology and adhesion on diamond films deposited and chemically modified by plasma processes

Popis výsledku v původním jazyce

The authors show that nanocrystalline diamond (NCD) thin films prepared by microwave plasma enhanced chemical vapor deposition apparatus with a linear antenna delivery system are well compatible with epithelial cells (5637 human bladder carcinoma) and significantly improve the cell adhesion compared to reference glass substrates. This is attributed to better adhesion of adsorbed layers to diamond as observed by atomic force microscopy (AFM) beneath the cells. Moreover, the cell morphology can be adjusted by appropriate surface treatment of diamond by using hydrogen and oxygen plasma. Cell bodies, cytoplasmic rims, and filopodia were characterized by Peakforce AFM. Oxidized NCD films perform better than other substrates under all conditions (96% of cells adhered well). A thin adsorbed layer formed from culture medium and supplemented with fetal bovine serum (FBS) covered the diamond surface and played an important role in the cell adhesion. Nevertheless, 50-100 nm large aggregates forme

Název v anglickém jazyce

Epithelial cell morphology and adhesion on diamond films deposited and chemically modified by plasma processes

Popis výsledku anglicky

The authors show that nanocrystalline diamond (NCD) thin films prepared by microwave plasma enhanced chemical vapor deposition apparatus with a linear antenna delivery system are well compatible with epithelial cells (5637 human bladder carcinoma) and significantly improve the cell adhesion compared to reference glass substrates. This is attributed to better adhesion of adsorbed layers to diamond as observed by atomic force microscopy (AFM) beneath the cells. Moreover, the cell morphology can be adjusted by appropriate surface treatment of diamond by using hydrogen and oxygen plasma. Cell bodies, cytoplasmic rims, and filopodia were characterized by Peakforce AFM. Oxidized NCD films perform better than other substrates under all conditions (96% of cells adhered well). A thin adsorbed layer formed from culture medium and supplemented with fetal bovine serum (FBS) covered the diamond surface and played an important role in the cell adhesion. Nevertheless, 50-100 nm large aggregates forme

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JI - Kompositní materiály

OECD FORD obor

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Projekt

<a href="/cs/project/KAN200100801" target="_blank" >KAN200100801: Bioaktivní biokompatibilní povrchy a nové nanostrukturované kompozity pro aplikace v medicíně a farmacii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biointerphases

ISSN

1934-8630

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

"Article ID 031012; 1-8"

Kód UT WoS článku

000340893400013

EID výsledku v databázi Scopus

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