Rapid determination of branched chain amino acids in human blood plasma by pressure assisted capillary electrophoresis with contactless conductivity detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43909342" target="_blank" >RIV/00216208:11120/15:43909342 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064173:_____/15:#0000489

Výsledek na webu

<a href="http://dx.doi.org/10.1002/elps.201400585" target="_blank" >http://dx.doi.org/10.1002/elps.201400585</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201400585" target="_blank" >10.1002/elps.201400585</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Rapid determination of branched chain amino acids in human blood plasma by pressure assisted capillary electrophoresis with contactless conductivity detection

Popis výsledku v původním jazyce

A capillary electrophoresis (CE) method with contactless conductivity detection has been developed for the clinical determination of the branched chain amino acids (BCAAs) valine, isoleucine, and leucine in human blood plasma. The CE separation was performed in an optimized background electrolyte with composition 3.2 M acetic acid in 20% v/v methanol, pH 2.0. The achieved separation time was 125 s when using a capillary with an effective length of 14.7 cm, electric field intensity of 0.96 kV/cm and simultaneous application of a hydrodynamic pressure of 50 mbar. The separation efficiency in blood plasma equalled 461 000 theoretical plates/m for valine and isoleucine, and 455 000 theoretical plates/m for leucine; the detection limits are equal to 0.4 ?Mfor all three amino acids. The relative standard deviation values for repeatability of the migration time equalled 0.1% for measurements during a single day and 0.3% for measurements on different days; the relative standard deviation valu

Název v anglickém jazyce

Rapid determination of branched chain amino acids in human blood plasma by pressure assisted capillary electrophoresis with contactless conductivity detection

Popis výsledku anglicky

A capillary electrophoresis (CE) method with contactless conductivity detection has been developed for the clinical determination of the branched chain amino acids (BCAAs) valine, isoleucine, and leucine in human blood plasma. The CE separation was performed in an optimized background electrolyte with composition 3.2 M acetic acid in 20% v/v methanol, pH 2.0. The achieved separation time was 125 s when using a capillary with an effective length of 14.7 cm, electric field intensity of 0.96 kV/cm and simultaneous application of a hydrodynamic pressure of 50 mbar. The separation efficiency in blood plasma equalled 461 000 theoretical plates/m for valine and isoleucine, and 455 000 theoretical plates/m for leucine; the detection limits are equal to 0.4 ?Mfor all three amino acids. The relative standard deviation values for repeatability of the migration time equalled 0.1% for measurements during a single day and 0.3% for measurements on different days; the relative standard deviation valu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/NT14416" target="_blank" >NT14416: Vliv větvených aminokyselin a saturovaných mastných kyselin na rozvoj inzulínové rezistence</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

16

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

1969-1975

Kód UT WoS článku

000360055300024

EID výsledku v databázi Scopus

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