Low noncarbonic buffer power amplifies acute respiratory acid-base disorders in patients with sepsis: an in vitro study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F21%3A43921730" target="_blank" >RIV/00216208:11120/21:43921730 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064173:_____/21:N0000049

Výsledek na webu

<a href="https://doi.org/10.1152/japplphysiol.00787.2020" target="_blank" >https://doi.org/10.1152/japplphysiol.00787.2020</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1152/japplphysiol.00787.2020" target="_blank" >10.1152/japplphysiol.00787.2020</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Low noncarbonic buffer power amplifies acute respiratory acid-base disorders in patients with sepsis: an in vitro study

Popis výsledku v původním jazyce

RATIONALE: Septic patients have typically reduced concentrations of hemoglobin and albumin, the major components of non-carbonic buffer power(β). This could expose patients to high pH variations during acid-base disorders. OBJECTIVES: To compare, in-vitro, non-carbonic β of septic patients with that of healthy volunteers, and evaluate its distinct components. METHODS: Whole blood and isolated plasma of 18 septic patients and 18 controls were equilibrated with different CO(2) mixtures. Blood gases, pH and electrolytes were measured. Non-carbonic β and non-carbonic β due to variations in Strong Ion Difference (β(SID)) were calculated for whole blood. Non-carbonic β and non-carbonic β normalized for albumin concentrations (β(NORM)) were calculated for isolated plasma. Representative values at pH=7.40 were compared. Albumin proteoforms were evaluated via two-dimensional electrophoresis. MEASUREMENTS AND MAIN RESULTS: Hemoglobin and albumin concentrations were significantly lower in septic patients. Septic patients had lower non-carbonic β both of whole blood (22.0+-1.9 vs. 31.6+-2.1 mmol/L, p&lt;0.01) and plasma (0.5+-1.0 vs. 3.7+-0.8 mmol/L, p&lt;0.01). Non-carbonic β(SID) was lower in patients (16.8+-1.9 vs. 24.4+-1.9 mmol/L, p&lt;0.01) and strongly correlated with hemoglobin concentration (r=0.94, p&lt;0.01). Non-carbonic β(NORM) was lower in patients (0.01 [-0.01 - 0.04] vs. 0.08 [0.06 - 0.09] mmol/g, p&lt;0.01). Septic patients and controls showed different amounts of albumin proteoforms. CONCLUSIONS: Septic patients are exposed to higher pH variations for any given change in CO(2) due to lower concentrations of non-carbonic buffers and, possibly, an altered buffering function of albumin. In both septic patients and healthy controls, electrolyte shifts are the major buffering mechanism during respiratory acid-base disorders.

Název v anglickém jazyce

Low noncarbonic buffer power amplifies acute respiratory acid-base disorders in patients with sepsis: an in vitro study

Popis výsledku anglicky

RATIONALE: Septic patients have typically reduced concentrations of hemoglobin and albumin, the major components of non-carbonic buffer power(β). This could expose patients to high pH variations during acid-base disorders. OBJECTIVES: To compare, in-vitro, non-carbonic β of septic patients with that of healthy volunteers, and evaluate its distinct components. METHODS: Whole blood and isolated plasma of 18 septic patients and 18 controls were equilibrated with different CO(2) mixtures. Blood gases, pH and electrolytes were measured. Non-carbonic β and non-carbonic β due to variations in Strong Ion Difference (β(SID)) were calculated for whole blood. Non-carbonic β and non-carbonic β normalized for albumin concentrations (β(NORM)) were calculated for isolated plasma. Representative values at pH=7.40 were compared. Albumin proteoforms were evaluated via two-dimensional electrophoresis. MEASUREMENTS AND MAIN RESULTS: Hemoglobin and albumin concentrations were significantly lower in septic patients. Septic patients had lower non-carbonic β both of whole blood (22.0+-1.9 vs. 31.6+-2.1 mmol/L, p&lt;0.01) and plasma (0.5+-1.0 vs. 3.7+-0.8 mmol/L, p&lt;0.01). Non-carbonic β(SID) was lower in patients (16.8+-1.9 vs. 24.4+-1.9 mmol/L, p&lt;0.01) and strongly correlated with hemoglobin concentration (r=0.94, p&lt;0.01). Non-carbonic β(NORM) was lower in patients (0.01 [-0.01 - 0.04] vs. 0.08 [0.06 - 0.09] mmol/g, p&lt;0.01). Septic patients and controls showed different amounts of albumin proteoforms. CONCLUSIONS: Septic patients are exposed to higher pH variations for any given change in CO(2) due to lower concentrations of non-carbonic buffers and, possibly, an altered buffering function of albumin. In both septic patients and healthy controls, electrolyte shifts are the major buffering mechanism during respiratory acid-base disorders.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30221 - Critical care medicine and Emergency medicine

Projekt

—

Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Physiology

ISSN

8750-7587

e-ISSN

—

Svazek periodika

131

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

464-473

Kód UT WoS článku

000693665100004

EID výsledku v databázi Scopus

2-s2.0-85113590461