The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft's Structural Damage and Mechanical Properties

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F20%3A10416917" target="_blank" >RIV/00216208:11130/20:10416917 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/20:00345861 RIV/00179906:_____/20:10416917 RIV/00023001:_____/20:00080309 RIV/00064165:_____/20:10416917

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=UJhWF3xME8" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=UJhWF3xME8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2020/6545190" target="_blank" >10.1155/2020/6545190</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft's Structural Damage and Mechanical Properties

Popis výsledku v původním jazyce

INTRODUCTION: The rate of thawing of cryopreserved human iliac arteries allografts (CHIAA) directly affects the severeness of structural changes that occur during this process. METHOD: The experiment was performed on ten CHIAA. The 10% dimethylsulphoxide in 6% hydroxyethyl starch solution was used as the cryoprotectant; all CHIAA were cooled at a controlled rate and stored in the vapor phase of liquid nitrogen (-194°C). Two thawing protocols were tested: (1) placing the CHIAA in a water bath at 37°C, and (2) the CHIAA were thawed in a controlled environment at 5°C. All samples underwent analysis under a scanning electron microscope. Testing of the mechanical properties of the CHIAA was evaluated on a custom-built single axis strain testing machine. Longitudinal and circumferential samples were prepared from each tested CHIAA. RESULTS: Ultrastructural analysis revealed that all five CHIAA thawed during the thawing protocol 1 which showed significantly more damage to the subendothelial structures when compared to the samples thawed in protocol 2. Mechanical properties: Thawing protocol 1-longitudinal UTS 2, 53 +- 0, 47 MPa at relative strain 1, 27 +- 0, 12 and circumferential UTS 1, 94 +- 0, 27 MPa at relative strain 1, 33 +- 0, 09. Thawing protocol 2-longitudinal ultimate tensile strain (UTS) 2, 42 +- 0, 34 MPa at relative strain 1, 32 +- 0, 09 and circumferential UTS 1, 98 +- 0, 26 MPa at relative strain 1, 29 +- 0, 07. Comparing UTS showed no statistical difference between thawing methods. CONCLUSION: Despite the significant differences in structural changes of presented thawing protocols, the ultimate tensile strain showed no statistical difference between thawing methods.

Název v anglickém jazyce

The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft's Structural Damage and Mechanical Properties

Popis výsledku anglicky

INTRODUCTION: The rate of thawing of cryopreserved human iliac arteries allografts (CHIAA) directly affects the severeness of structural changes that occur during this process. METHOD: The experiment was performed on ten CHIAA. The 10% dimethylsulphoxide in 6% hydroxyethyl starch solution was used as the cryoprotectant; all CHIAA were cooled at a controlled rate and stored in the vapor phase of liquid nitrogen (-194°C). Two thawing protocols were tested: (1) placing the CHIAA in a water bath at 37°C, and (2) the CHIAA were thawed in a controlled environment at 5°C. All samples underwent analysis under a scanning electron microscope. Testing of the mechanical properties of the CHIAA was evaluated on a custom-built single axis strain testing machine. Longitudinal and circumferential samples were prepared from each tested CHIAA. RESULTS: Ultrastructural analysis revealed that all five CHIAA thawed during the thawing protocol 1 which showed significantly more damage to the subendothelial structures when compared to the samples thawed in protocol 2. Mechanical properties: Thawing protocol 1-longitudinal UTS 2, 53 +- 0, 47 MPa at relative strain 1, 27 +- 0, 12 and circumferential UTS 1, 94 +- 0, 27 MPa at relative strain 1, 33 +- 0, 09. Thawing protocol 2-longitudinal ultimate tensile strain (UTS) 2, 42 +- 0, 34 MPa at relative strain 1, 32 +- 0, 09 and circumferential UTS 1, 98 +- 0, 26 MPa at relative strain 1, 29 +- 0, 07. Comparing UTS showed no statistical difference between thawing methods. CONCLUSION: Despite the significant differences in structural changes of presented thawing protocols, the ultimate tensile strain showed no statistical difference between thawing methods.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30106 - Anatomy and morphology (plant science to be 1.6)

Projekt

<a href="/cs/project/NV18-02-00422" target="_blank" >NV18-02-00422: Nové materiály pro kardiovaskulární chirurgii na bázi modifikovaných decelularizovaných tkání</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BioMed Research International

ISSN

2314-6133

e-ISSN

—

Svazek periodika

2020

Číslo periodika v rámci svazku

October

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

6545190

Kód UT WoS článku

000585705300001

EID výsledku v databázi Scopus

2-s2.0-85094655715