Gastric submucosal microdialysis in detection of rat stomach ischemia - a comparison of the 3H2O efflux technique with metabolic monitoring
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F10%3A10062016" target="_blank" >RIV/00216208:11150/10:10062016 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61989592:15110/10:10213168 RIV/00179906:_____/10:10062016
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Gastric submucosal microdialysis in detection of rat stomach ischemia - a comparison of the 3H2O efflux technique with metabolic monitoring
Popis výsledku v původním jazyce
Comparison of microdialysis approaches to measure blood flow in the stomach is lacking. We compared 3H2O efflux technique with biochemical monitoring during temporary celiac artery occlusion in rats. Probes were implanted in the gastric submucosa and perfused with 3H2O; samples were analyzed for beta-activity, glucose, lactate, pyruvate and glycerol. Gastric mucosa and plasma were subjected to morphometry and analysis of myeloperoxidase, thiols and lactatdehydrogenase. The most dramatic responses were observed in lactate/pyruvate and lactate/glucose (%) ratios; the change in 3H2O efflux was less pronounced. 3H2O efflux correlated best with lactate/glucose ratio. A correlation was also found between plasma lactatdehydrogenase and relative glycerol release. Myeloperoxidase, lactatdehydrogenase and histology score were increased by ischemia-reperfusion, macroscopy and plasma thiols remained unchanged. Microdialysis metabolic monitoring is superior to the 3H2O efflux technique.
Název v anglickém jazyce
Gastric submucosal microdialysis in detection of rat stomach ischemia - a comparison of the 3H2O efflux technique with metabolic monitoring
Popis výsledku anglicky
Comparison of microdialysis approaches to measure blood flow in the stomach is lacking. We compared 3H2O efflux technique with biochemical monitoring during temporary celiac artery occlusion in rats. Probes were implanted in the gastric submucosa and perfused with 3H2O; samples were analyzed for beta-activity, glucose, lactate, pyruvate and glycerol. Gastric mucosa and plasma were subjected to morphometry and analysis of myeloperoxidase, thiols and lactatdehydrogenase. The most dramatic responses were observed in lactate/pyruvate and lactate/glucose (%) ratios; the change in 3H2O efflux was less pronounced. 3H2O efflux correlated best with lactate/glucose ratio. A correlation was also found between plasma lactatdehydrogenase and relative glycerol release. Myeloperoxidase, lactatdehydrogenase and histology score were increased by ischemia-reperfusion, macroscopy and plasma thiols remained unchanged. Microdialysis metabolic monitoring is superior to the 3H2O efflux technique.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FP - Ostatní lékařské obory
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Physiological Measurement
ISSN
0967-3334
e-ISSN
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Svazek periodika
31
Číslo periodika v rámci svazku
10
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
14
Strana od-do
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Kód UT WoS článku
000281726900009
EID výsledku v databázi Scopus
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