Multispecies biofilm in an artificial wound bed-A novel model for in vitro assessment of solid antimicrobial dressings

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F14%3A10284030" target="_blank" >RIV/00216208:11150/14:10284030 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/14:43908522

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.mimet.2014.05.008" target="_blank" >http://dx.doi.org/10.1016/j.mimet.2014.05.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mimet.2014.05.008" target="_blank" >10.1016/j.mimet.2014.05.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multispecies biofilm in an artificial wound bed-A novel model for in vitro assessment of solid antimicrobial dressings

Popis výsledku v původním jazyce

Wound infections represent a major problem, particularly in patients with chronic wounds. Bacteria in the wound exist mainly in the form of biofilms and are thus resistant to most antibiotics and antimicrobials. A simple and cost-effective in vitro modelof chronic wound biofilms applied for testing treatments and solid devices, especially wound dressings, is presented in this work. The method is based on the well-established Lubbock chronic wound biofilm transferred onto an artificial agar wound bed. The biofilm formed by four bacterial species (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa) was stable for up to 48 h post-transplant. The applicability of the model was evaluated by testing two common iodinewound treatments. These observations indicate that this method enables assessing the effects of treatments on established resilient wound biofilms and is clinically highly relevant. (C) 2014 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Multispecies biofilm in an artificial wound bed-A novel model for in vitro assessment of solid antimicrobial dressings

Popis výsledku anglicky

Wound infections represent a major problem, particularly in patients with chronic wounds. Bacteria in the wound exist mainly in the form of biofilms and are thus resistant to most antibiotics and antimicrobials. A simple and cost-effective in vitro modelof chronic wound biofilms applied for testing treatments and solid devices, especially wound dressings, is presented in this work. The method is based on the well-established Lubbock chronic wound biofilm transferred onto an artificial agar wound bed. The biofilm formed by four bacterial species (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa) was stable for up to 48 h post-transplant. The applicability of the model was evaluated by testing two common iodinewound treatments. These observations indicate that this method enables assessing the effects of treatments on established resilient wound biofilms and is clinically highly relevant. (C) 2014 Elsevier B.V. All rights reserved.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiological Methods

ISSN

0167-7012

e-ISSN

—

Svazek periodika

103

Číslo periodika v rámci svazku

August

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

18-24

Kód UT WoS článku

000339150300005

EID výsledku v databázi Scopus

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