Multispecies biofilm in an artificial wound bed—A novel model for in vitro assessment of solid antimicrobial dressings

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60917431%3A_____%2F14%3A00TA2902" target="_blank" >RIV/60917431:_____/14:00TA2902 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0167701214001304?np=y" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0167701214001304?np=y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mimet.2014.05.008" target="_blank" >10.1016/j.mimet.2014.05.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multispecies biofilm in an artificial wound bed—A novel model for in vitro assessment of solid antimicrobial dressings

Popis výsledku v původním jazyce

Wound infections represent a major problem, particularly in patients with chronic wounds. Bacteria in the wound exist mainly in the formof biofilms and are thus resistant to most antibiotics and antimicrobials. A simple and cost-effective in vitro model of chronic wound biofilms applied for testing treatments and solid devices, especially wound dressings, is presented in this work. The method is based on the well-established Lubbock chronic wound biofilm transferred onto an artificial agar wound bed. The biofilm formed by four bacterial species (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa) was stable for up to 48 h post-transplant. The applicability of the model was evaluated by testing two common iodine wound treatments. These observations indicate that this method enables assessing the effects of treatments on established resilient wound biofilms and is clinically highly relevant.

Název v anglickém jazyce

Multispecies biofilm in an artificial wound bed—A novel model for in vitro assessment of solid antimicrobial dressings

Popis výsledku anglicky

Wound infections represent a major problem, particularly in patients with chronic wounds. Bacteria in the wound exist mainly in the formof biofilms and are thus resistant to most antibiotics and antimicrobials. A simple and cost-effective in vitro model of chronic wound biofilms applied for testing treatments and solid devices, especially wound dressings, is presented in this work. The method is based on the well-established Lubbock chronic wound biofilm transferred onto an artificial agar wound bed. The biofilm formed by four bacterial species (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa) was stable for up to 48 h post-transplant. The applicability of the model was evaluated by testing two common iodine wound treatments. These observations indicate that this method enables assessing the effects of treatments on established resilient wound biofilms and is clinically highly relevant.

Druh

O - Ostatní výsledky

CEP obor

FO - Dermatovenerologie

OECD FORD obor

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Projekt

<a href="/cs/project/TA03011029" target="_blank" >TA03011029: Nové kryty ran s programovaným uvolňováním účinných látek určené pro inhibici biofilmu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.