Comet assay in evaluating deoxyribonucleic acid damage after out-of-hospital cardiac arrest

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F17%3A10363516" target="_blank" >RIV/00216208:11150/17:10363516 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00179906:_____/17:10363516

Výsledek na webu

<a href="http://dx.doi.org/10.14744/AnatolJCardiol.2017.7578" target="_blank" >http://dx.doi.org/10.14744/AnatolJCardiol.2017.7578</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14744/AnatolJCardiol.2017.7578" target="_blank" >10.14744/AnatolJCardiol.2017.7578</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comet assay in evaluating deoxyribonucleic acid damage after out-of-hospital cardiac arrest

Popis výsledku v původním jazyce

Objective: This study aimed to investigate whether out-of-hospital cardiac arrest (OHCA) may induce severe DNA damage measured using comet assay in successfully resuscitated humans and to evaluate a short-term prognostic role. Methods: In this prospective, controlled, blinded study (1/2013-1/2014), 41 patients (age, 63 +/- 14 years) successfully resuscitated from non-traumatic OHCA and 10 healthy controls (age, 53 +/- 17 years) were enrolled. DNA damage [double-strand breaks (DSBs) and single-strand breaks (SSBs)] was measured using comet assay in peripheral lymphocytes sampled at admission. Clinical data were recorded (according to Utstein style). A good short-term prognosis was defined as survival for 30 days. Results: Among the patients, there were 71% (29/41) short-term survivors. After OHCA, DNA damage (DSBs and SSBs) was higher (11.0 +/- 7.6% and 0.79 +/- 2.41% in tail) among patients than among controls (1.96 +/- 1.63% and 0.02 +/- 0.03% in tail), and it was more apparent for DSBs (p&lt; 0.001 and p=0.085). There was no difference in the DNA damage between patients with cardiac and non-cardiac etiology, or between survivors and nonsurvivors. Among Utstein style parameters, ventricular fibrillation, asystole, and early electrical defibrillation influenced DSBs; none of the factors influenced SSBs. Factors influencing survival were SSBs, ventricular fibrillation, length of cardiopulmonary resuscitation by professionals =15 min, cardiogenic shock, and postanoxic encephalopathy. In contrast to DSBs [area under the curve (AUC)=0.520], SSBs seem to have a potential in prognostication (AUC=0.639). Conclusion: This study for the first time demonstrates revelation of DNA damage using comet assay in patients successfully resuscitated from OHCA. Whether DNA damage measured using comet assay may be a prognostic marker remains unknown, although our data may encourage some suggestions.

Název v anglickém jazyce

Comet assay in evaluating deoxyribonucleic acid damage after out-of-hospital cardiac arrest

Popis výsledku anglicky

Objective: This study aimed to investigate whether out-of-hospital cardiac arrest (OHCA) may induce severe DNA damage measured using comet assay in successfully resuscitated humans and to evaluate a short-term prognostic role. Methods: In this prospective, controlled, blinded study (1/2013-1/2014), 41 patients (age, 63 +/- 14 years) successfully resuscitated from non-traumatic OHCA and 10 healthy controls (age, 53 +/- 17 years) were enrolled. DNA damage [double-strand breaks (DSBs) and single-strand breaks (SSBs)] was measured using comet assay in peripheral lymphocytes sampled at admission. Clinical data were recorded (according to Utstein style). A good short-term prognosis was defined as survival for 30 days. Results: Among the patients, there were 71% (29/41) short-term survivors. After OHCA, DNA damage (DSBs and SSBs) was higher (11.0 +/- 7.6% and 0.79 +/- 2.41% in tail) among patients than among controls (1.96 +/- 1.63% and 0.02 +/- 0.03% in tail), and it was more apparent for DSBs (p&lt; 0.001 and p=0.085). There was no difference in the DNA damage between patients with cardiac and non-cardiac etiology, or between survivors and nonsurvivors. Among Utstein style parameters, ventricular fibrillation, asystole, and early electrical defibrillation influenced DSBs; none of the factors influenced SSBs. Factors influencing survival were SSBs, ventricular fibrillation, length of cardiopulmonary resuscitation by professionals =15 min, cardiogenic shock, and postanoxic encephalopathy. In contrast to DSBs [area under the curve (AUC)=0.520], SSBs seem to have a potential in prognostication (AUC=0.639). Conclusion: This study for the first time demonstrates revelation of DNA damage using comet assay in patients successfully resuscitated from OHCA. Whether DNA damage measured using comet assay may be a prognostic marker remains unknown, although our data may encourage some suggestions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30201 - Cardiac and Cardiovascular systems

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Anatolian Journal of Cardiology

ISSN

2149-2263

e-ISSN

—

Svazek periodika

18

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

TR - Turecká republika

Počet stran výsledku

8

Strana od-do

31-38

Kód UT WoS článku

000406137400007

EID výsledku v databázi Scopus

2-s2.0-85021723478