Chromatographic method for the determination of inflammatory biomarkers and uric acid in human saliva

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F21%3A10428601" target="_blank" >RIV/00216208:11150/21:10428601 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00179906:_____/21:10428601 RIV/00216208:11160/21:10428601 RIV/61989592:15110/21:73608586

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=t9pOMQTZiz" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=t9pOMQTZiz</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2021.122598" target="_blank" >10.1016/j.talanta.2021.122598</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chromatographic method for the determination of inflammatory biomarkers and uric acid in human saliva

Popis výsledku v původním jazyce

Determination of concentration of biomarkers of the activation of immune system, uric acid, and creatinine in the saliva can be useful tool for the diagnosis and monitoring of early manifestations of diseases such as malignant, inflammatory, and periodontal disorders. We have developed and validated a high-performance liquid chromatographic method coupled with fluorescence and diode array detection for the separation and quantification of neopterin, tryptophan, creatinine, uric acid, and kynurenine in the human saliva. A separation of these analytes was achieved within 9 min by using second-generation monolithic stationary phase and elution with phosphate buffer. The present method involves very simple sample preparation requiring small amount of sample matrix. The internal standard 3-nitro-L-tyrosine was used for a more precise quantification. The sensitivity of the present method was demonstrated with lower limits of quantification of 0.6 x 10(-3) mu mol/L for neopterin, 0.725 mu mol/L for tryptophan, 0.12 mu mol/L for creatinine, 0.18 mu mol/L for uric acid, and 0.135 mu mol/L for kynurenine. The method was validated with 67 real-life saliva samples collected from patients suffering from breast, ovarian, colorectal, and renal cancer, and 19 saliva samples from patients with periodontal diseases and allowed monitoring of inflammatory response.

Název v anglickém jazyce

Chromatographic method for the determination of inflammatory biomarkers and uric acid in human saliva

Popis výsledku anglicky

Determination of concentration of biomarkers of the activation of immune system, uric acid, and creatinine in the saliva can be useful tool for the diagnosis and monitoring of early manifestations of diseases such as malignant, inflammatory, and periodontal disorders. We have developed and validated a high-performance liquid chromatographic method coupled with fluorescence and diode array detection for the separation and quantification of neopterin, tryptophan, creatinine, uric acid, and kynurenine in the human saliva. A separation of these analytes was achieved within 9 min by using second-generation monolithic stationary phase and elution with phosphate buffer. The present method involves very simple sample preparation requiring small amount of sample matrix. The internal standard 3-nitro-L-tyrosine was used for a more precise quantification. The sensitivity of the present method was demonstrated with lower limits of quantification of 0.6 x 10(-3) mu mol/L for neopterin, 0.725 mu mol/L for tryptophan, 0.12 mu mol/L for creatinine, 0.18 mu mol/L for uric acid, and 0.135 mu mol/L for kynurenine. The method was validated with 67 real-life saliva samples collected from patients suffering from breast, ovarian, colorectal, and renal cancer, and 19 saliva samples from patients with periodontal diseases and allowed monitoring of inflammatory response.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

—

Svazek periodika

233

Číslo periodika v rámci svazku

October

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

122598

Kód UT WoS článku

000668000500103

EID výsledku v databázi Scopus

2-s2.0-85107824803