Fast and sensitive HPLC method for the determination of neopterin, kynurenine and tryptophan in amniotic fluid, malignant effusions and wound exudates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33155485" target="_blank" >RIV/61989592:15110/15:33155485 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/15:10312560 RIV/00179906:_____/15:10312560

Výsledek na webu

<a href="http://dx.doi.org/10.4155/bio.15.175" target="_blank" >http://dx.doi.org/10.4155/bio.15.175</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4155/bio.15.175" target="_blank" >10.4155/bio.15.175</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fast and sensitive HPLC method for the determination of neopterin, kynurenine and tryptophan in amniotic fluid, malignant effusions and wound exudates

Popis výsledku v původním jazyce

Aim: A new HPLC method for the determination of neopterin, kynurenine and tryptophan using a second-generation monolith stationary phase and high-throughput sample preparation procedure based on microplates was developed and fully validated. Materials &methods: As the stationary phase a monolithic C18 Chromolith high-resolution column with dimensions of 4.6 x 100 mm connected to a monolithic 4.6 x 10-mm security guard was used. Separation was achieved using 15 mM phosphate buffer (KH2PO4 + K2HPO4 center dot 3H(2)O at pH 3) and acetonitrile in gradient mode. Results: Target analytes were determined in 5.5 minutes in amniotic fluid, effusions and wound exudates with a limit of quantification (LOQ) of 1.25 nM for neopterin, 2.5 mu M for tryptophan and 0.25 mu M for kynurenine. Discussion: The method was applied to real clinical sample measurements, and it will be used to monitor neopterin, kynurenine and tryptophan levels in biological fluids to assess the patient response to therapy and

Název v anglickém jazyce

Fast and sensitive HPLC method for the determination of neopterin, kynurenine and tryptophan in amniotic fluid, malignant effusions and wound exudates

Popis výsledku anglicky

Aim: A new HPLC method for the determination of neopterin, kynurenine and tryptophan using a second-generation monolith stationary phase and high-throughput sample preparation procedure based on microplates was developed and fully validated. Materials &methods: As the stationary phase a monolithic C18 Chromolith high-resolution column with dimensions of 4.6 x 100 mm connected to a monolithic 4.6 x 10-mm security guard was used. Separation was achieved using 15 mM phosphate buffer (KH2PO4 + K2HPO4 center dot 3H(2)O at pH 3) and acetonitrile in gradient mode. Results: Target analytes were determined in 5.5 minutes in amniotic fluid, effusions and wound exudates with a limit of quantification (LOQ) of 1.25 nM for neopterin, 2.5 mu M for tryptophan and 0.25 mu M for kynurenine. Discussion: The method was applied to real clinical sample measurements, and it will be used to monitor neopterin, kynurenine and tryptophan levels in biological fluids to assess the patient response to therapy and

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bioanalysis

ISSN

1757-6180

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

21

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

2751-2762

Kód UT WoS článku

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EID výsledku v databázi Scopus

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