Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F11%3A10223934" target="_blank" >RIV/61989592:15110/11:10223934 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/11:10100325 RIV/00216208:11160/11:10100325 RIV/00179906:_____/11:10100325

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC

Popis výsledku v původním jazyce

A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithiccartridge 4.6 mm x 50 mm + 3.0 mm x 100 mm and 4.6 x 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice. (C) 2011 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC

Popis výsledku anglicky

A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithiccartridge 4.6 mm x 50 mm + 3.0 mm x 100 mm and 4.6 x 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice. (C) 2011 Elsevier B.V. All rights reserved.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

TALANTA

ISSN

0039-9140

e-ISSN

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Svazek periodika

85

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

1466-1471

Kód UT WoS článku

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EID výsledku v databázi Scopus

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