Chromatographic determination of active compounds in topical formulations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F12%3A10124373" target="_blank" >RIV/00216208:11160/12:10124373 - isvavai.cz</a>

Výsledek na webu

<a href="http://pubs.rsc.org/en/content/articlepdf/2011/ay/c1ay05336a" target="_blank" >http://pubs.rsc.org/en/content/articlepdf/2011/ay/c1ay05336a</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c1ay05336a" target="_blank" >10.1039/c1ay05336a</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chromatographic determination of active compounds in topical formulations

Popis výsledku v původním jazyce

The aim of this study was to develop and validate new methods for the determination of active compounds in topical gels. The method for the determination of nonoxinol-9 and trimecaine hydrochloride in lubricant gel by HPLC with UV detection was described. Separation was carried out using a Discovery ZR-PBD analytical column (150 x 4.6 mm, 5 mu m), and a mobile phase composed of a mixture of triethylammonium acetate buffer (pH 9; 50 mM) and methanol (22 : 78, v/v). Determination of terpinen-4-ol in lubricant gel was achieved by GC with a flame ionization detector (FID). An Alltech-AT-624 (0.32 mm ID x 30 m, 1.8 mu m film thickness) fused-silica capillary column and helium as the carrier gas, at a flow rate of 28 cm s(-1), were employed. The analysis time was less than 7.5 min for HPLC and 5 min for GC analyses. Both methods were successfully applied to the analysis of pharmaceuticals.

Název v anglickém jazyce

Chromatographic determination of active compounds in topical formulations

Popis výsledku anglicky

The aim of this study was to develop and validate new methods for the determination of active compounds in topical gels. The method for the determination of nonoxinol-9 and trimecaine hydrochloride in lubricant gel by HPLC with UV detection was described. Separation was carried out using a Discovery ZR-PBD analytical column (150 x 4.6 mm, 5 mu m), and a mobile phase composed of a mixture of triethylammonium acetate buffer (pH 9; 50 mM) and methanol (22 : 78, v/v). Determination of terpinen-4-ol in lubricant gel was achieved by GC with a flame ionization detector (FID). An Alltech-AT-624 (0.32 mm ID x 30 m, 1.8 mu m film thickness) fused-silica capillary column and helium as the carrier gas, at a flow rate of 28 cm s(-1), were employed. The analysis time was less than 7.5 min for HPLC and 5 min for GC analyses. Both methods were successfully applied to the analysis of pharmaceuticals.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Methods

ISSN

1759-9660

e-ISSN

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Svazek periodika

4

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

1525-1529

Kód UT WoS článku

000304768600010

EID výsledku v databázi Scopus

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