A New and Fast HPLC Method for Determination of Rutin, Troxerutin, Diosmin and Hesperidin in Food Supplements Using Fused-Core Column Technology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10146034" target="_blank" >RIV/00216208:11160/13:10146034 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007/s12161-012-9551-y/fulltext.html" target="_blank" >http://link.springer.com/article/10.1007/s12161-012-9551-y/fulltext.html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12161-012-9551-y" target="_blank" >10.1007/s12161-012-9551-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A New and Fast HPLC Method for Determination of Rutin, Troxerutin, Diosmin and Hesperidin in Food Supplements Using Fused-Core Column Technology

Popis výsledku v původním jazyce

A new and fast high-performance liquid chromatography (HPLC) method using fused-core column for separation of rutin, troxerutin, diosmin, and hesperidin has been developed and used for determination of these flavonoids in food supplements. Efficient separation of flavonoids and internal standard methylparaben was achieved on the fused-core column Ascentis Express RP-Amide (100 x 3.0 mm), particle size 2.7 mu m, with mobile phase acetonitrile/water solution of acetic acid pH 3 (30:70, v/v) at a flow rateof 1.0 mL min(-1) and at temperature 50 A degrees C. The detection wavelengths were set at 283 nm for hesperidin and at 255 nm for rutin, troxerutin, diosmin, and internal standard methylparaben. Under the optimal chromatographic conditions, good linearity with correlation coefficients in the range (r = 0.9991-0.9998; n = 7) for all flavonoids was achieved. Commercial samples of food supplements were extracted with 100 % dimethyl sulfoxide using ultrasound bath for 10 min and then dilut

Název v anglickém jazyce

A New and Fast HPLC Method for Determination of Rutin, Troxerutin, Diosmin and Hesperidin in Food Supplements Using Fused-Core Column Technology

Popis výsledku anglicky

A new and fast high-performance liquid chromatography (HPLC) method using fused-core column for separation of rutin, troxerutin, diosmin, and hesperidin has been developed and used for determination of these flavonoids in food supplements. Efficient separation of flavonoids and internal standard methylparaben was achieved on the fused-core column Ascentis Express RP-Amide (100 x 3.0 mm), particle size 2.7 mu m, with mobile phase acetonitrile/water solution of acetic acid pH 3 (30:70, v/v) at a flow rateof 1.0 mL min(-1) and at temperature 50 A degrees C. The detection wavelengths were set at 283 nm for hesperidin and at 255 nm for rutin, troxerutin, diosmin, and internal standard methylparaben. Under the optimal chromatographic conditions, good linearity with correlation coefficients in the range (r = 0.9991-0.9998; n = 7) for all flavonoids was achieved. Commercial samples of food supplements were extracted with 100 % dimethyl sulfoxide using ultrasound bath for 10 min and then dilut

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Analytical Methods

ISSN

1936-9751

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

1353-1360

Kód UT WoS článku

000324271200014

EID výsledku v databázi Scopus

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