A Fast HPLC Method for Determination of Vitamin E Acetate in Dietary Supplements Using Monolithic Column

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10146035" target="_blank" >RIV/00216208:11160/13:10146035 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007/s12161-012-9452-0/fulltext.html" target="_blank" >http://link.springer.com/article/10.1007/s12161-012-9452-0/fulltext.html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12161-012-9452-0" target="_blank" >10.1007/s12161-012-9452-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Fast HPLC Method for Determination of Vitamin E Acetate in Dietary Supplements Using Monolithic Column

Popis výsledku v původním jazyce

A simple and fast high-performance liquid chromatography (HPLC) method with rapid separation on monolithic column has been developed for quantitative determination of vitamin E acetate in dietary supplements. Commercial samples of dietary supplements were extracted with 100 % methanol with the help of ultrasound bath. A 20-mu L sample volume of the filtered solution was directly injected into the HPLC system. Separation of ballast matrix, vitamin E acetate, and internal standard cholecalciferol was performed on the monolithic column Chromolith Performance RP-18e column (100 x 4.6 mm) with mobile phase methanol/water (98:2, v/v) at a flow rate of 2.0 mL min(-1) and at temperature 30 A degrees C. The detector was set at 290 nm. Under the optimum chromatographic conditions, standard calibration curve was measured with good linearity-correlation coefficient for vitamin E acetate (r = 0.9992; n = 6) between the peak areas and concentrations of vitamin E acetate. Accuracy of the method defin

Název v anglickém jazyce

A Fast HPLC Method for Determination of Vitamin E Acetate in Dietary Supplements Using Monolithic Column

Popis výsledku anglicky

A simple and fast high-performance liquid chromatography (HPLC) method with rapid separation on monolithic column has been developed for quantitative determination of vitamin E acetate in dietary supplements. Commercial samples of dietary supplements were extracted with 100 % methanol with the help of ultrasound bath. A 20-mu L sample volume of the filtered solution was directly injected into the HPLC system. Separation of ballast matrix, vitamin E acetate, and internal standard cholecalciferol was performed on the monolithic column Chromolith Performance RP-18e column (100 x 4.6 mm) with mobile phase methanol/water (98:2, v/v) at a flow rate of 2.0 mL min(-1) and at temperature 30 A degrees C. The detector was set at 290 nm. Under the optimum chromatographic conditions, standard calibration curve was measured with good linearity-correlation coefficient for vitamin E acetate (r = 0.9992; n = 6) between the peak areas and concentrations of vitamin E acetate. Accuracy of the method defin

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Analytical Methods

ISSN

1936-9751

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

380-385

Kód UT WoS článku

000316291500004

EID výsledku v databázi Scopus

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