Micellar electrokinetic chromatography with short-end injection for rapid separation and simultaneous determination of aesculin, aesculetin, and phenylephrine in pharmaceutical preparations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F15%3A10317293" target="_blank" >RIV/00216208:11160/15:10317293 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/elps.201500215/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/elps.201500215/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201500215" target="_blank" >10.1002/elps.201500215</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Micellar electrokinetic chromatography with short-end injection for rapid separation and simultaneous determination of aesculin, aesculetin, and phenylephrine in pharmaceutical preparations

Popis výsledku v původním jazyce

A fast micellar electrokinetic chromatography (MEKC) method for simultaneous assay of aesculin, aesculetin, and phenylephrine was developed and validated. The separation was carried out in a fused-silica capillary (50 mu m id, total length 64.5 cm, effective length 8.5 cm) with UV detection at 210 nm, temperature 25 degrees C and separation voltage -25 kV. The samples were loaded hydrodynamically at a pressure of -50 mbar for 6 s. The background electrolyte of pH 8.6 contained 20 mM boric acid, 60 mM SDS, and 5% (v/v) of methanol. The calibration curves were linear in the range 10-500 mu g/mL for aesculin and aesculetin and 12.5-625 mu g/mL for phenylephrine. The RSD values of corrected peak areas were 0.6-1.2% (n = 6) when determining 0.2 mg/mL of aesculin and aesculetin and 0.25 mg/mL of phenylephrine in prepared standard mixtures. The method was successfully applied to the assay of aesculin and phenylephrine in a pharmaceutical preparation (RSD = 1.9-2.0%; n = 3) and the robustness

Název v anglickém jazyce

Micellar electrokinetic chromatography with short-end injection for rapid separation and simultaneous determination of aesculin, aesculetin, and phenylephrine in pharmaceutical preparations

Popis výsledku anglicky

A fast micellar electrokinetic chromatography (MEKC) method for simultaneous assay of aesculin, aesculetin, and phenylephrine was developed and validated. The separation was carried out in a fused-silica capillary (50 mu m id, total length 64.5 cm, effective length 8.5 cm) with UV detection at 210 nm, temperature 25 degrees C and separation voltage -25 kV. The samples were loaded hydrodynamically at a pressure of -50 mbar for 6 s. The background electrolyte of pH 8.6 contained 20 mM boric acid, 60 mM SDS, and 5% (v/v) of methanol. The calibration curves were linear in the range 10-500 mu g/mL for aesculin and aesculetin and 12.5-625 mu g/mL for phenylephrine. The RSD values of corrected peak areas were 0.6-1.2% (n = 6) when determining 0.2 mg/mL of aesculin and aesculetin and 0.25 mg/mL of phenylephrine in prepared standard mixtures. The method was successfully applied to the assay of aesculin and phenylephrine in a pharmaceutical preparation (RSD = 1.9-2.0%; n = 3) and the robustness

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/EE2.3.30.0061" target="_blank" >EE2.3.30.0061: Zvýšení kapacity vědecko-výzkumných týmů Univerzity Karlovy prostřednictvím nových pozic pro absolventy doktorandských studií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Svazek periodika

36

Číslo periodika v rámci svazku

21

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

2754-2761

Kód UT WoS článku

000365620500015

EID výsledku v databázi Scopus

2-s2.0-84947866754