Automated Sequential Injection Method for Determination of Caffeine in Coffee Drinks

Kód výsledku v IS VaVaI

RIV/00216208:11160/18:10382499 - isvavai.cz

Výsledek na webu

http://link.springer.com/article/10.1007/s12161-017-0982-3

DOI - Digital Object Identifier

10.1007/s12161-017-0982-3

Jazyk výsledku

angličtina

Název v původním jazyce

Automated Sequential Injection Method for Determination of Caffeine in Coffee Drinks

Popis výsledku v původním jazyce

An automated sequential injection analysis spectrophotometric assay for the determination of purine alkaloids in coffee drinks was developed. The sample was treated with a carrez reagent for matrix suppression followed by filtration; subsequently, alkaloids were separated from organic acids using a short C18 monolithic column (10 x 4.6 mm). The flow rate of the separation step was 10 mu L s(-1) with 10% v/v of methanol as the mobile phase. The sum of alkaloids evaluated as caffeine was detected at 274 nm. The influence of the main parameters affecting the quantification of purine alkaloids was optimized. One sample analysis lasted 15 min when aspirated in triplicate. The linear range was 1-15 mg L-1, and the determination coefficient (r (2)) was 0.9969. The limit of detection and limit of quantitation were 0.128 and 0.425 mg L-1, respectively. The repeatability evaluated as the relative standard deviation (RSD) was 3.58% (n = 12, 10 mg L-1). Under optimal conditions, the method was successfully applied to determine purine alkaloids in different real samples including soluble coffee, coffee from an espresso machine, and brewed coffee drinks.

Název v anglickém jazyce

Automated Sequential Injection Method for Determination of Caffeine in Coffee Drinks

Popis výsledku anglicky

An automated sequential injection analysis spectrophotometric assay for the determination of purine alkaloids in coffee drinks was developed. The sample was treated with a carrez reagent for matrix suppression followed by filtration; subsequently, alkaloids were separated from organic acids using a short C18 monolithic column (10 x 4.6 mm). The flow rate of the separation step was 10 mu L s(-1) with 10% v/v of methanol as the mobile phase. The sum of alkaloids evaluated as caffeine was detected at 274 nm. The influence of the main parameters affecting the quantification of purine alkaloids was optimized. One sample analysis lasted 15 min when aspirated in triplicate. The linear range was 1-15 mg L-1, and the determination coefficient (r (2)) was 0.9969. The limit of detection and limit of quantitation were 0.128 and 0.425 mg L-1, respectively. The repeatability evaluated as the relative standard deviation (RSD) was 3.58% (n = 12, 10 mg L-1). Under optimal conditions, the method was successfully applied to determine purine alkaloids in different real samples including soluble coffee, coffee from an espresso machine, and brewed coffee drinks.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

EF15_003/0000465: Vytvoření expertního týmu pro pokročilý výzkum v separačních vědách

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
S - Specificky vyzkum na vysokych skolach
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Analytical Methods

ISSN

1936-9751

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

111-118

Kód UT WoS článku

000418836200012

EID výsledku v databázi Scopus

2-s2.0-85021801331