Fully automated method based on on-line molecularly imprinted polymer solid-phase extraction for determination of lovastatin in dietary supplements containing red yeast rice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F19%3A10399563" target="_blank" >RIV/00216208:11160/19:10399563 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=B2aw_d6Zvo" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=B2aw_d6Zvo</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-018-1554-0" target="_blank" >10.1007/s00216-018-1554-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fully automated method based on on-line molecularly imprinted polymer solid-phase extraction for determination of lovastatin in dietary supplements containing red yeast rice

Popis výsledku v původním jazyce

A fully automated method for the determination of lovastatin in dietary supplements containing red yeast rice has been developed. It uses a sequential injection analysis system combined with solid-phase extraction applying highly selective molecularly imprinted polymer sorbent. A miniaturized column for on-line extraction was prepared by packing 4.5mg of the sorbent in a 5.0x2.5-mm-i.d. cartridge, which was used in the flow manifold. Sequential injection analysis manifold enabled all steps of lovastatin extraction and continuous spectrophotometric detection at 240nm. A limit of detection of 60 mu g g(-1), a limit of quantitation of 200 mu g g(-1), and a linear calibration range of 200-2000 mu g g(-1) were achieved. Intra-day and inter-day precision values (RSD) were &lt;=6.7% and &lt;=4.9%, respectively, and method recovery values of spiked red yeast rice extracts at 200, 1000, and 2000 mu g g(-1) concentration levels were 82.9, 95.2, and 87.7%. Our method was used for determination of lovastatin lactone in four dietary supplements containing red yeast rice as a natural source of lovastatin, also known as monacolin K. The extracted samples were subsequently analyzed by the reference UHPLC-MS/MS method. Statistical comparison of results (F test, t test, alpha=0.05) obtained by both methods did not reveal significant difference. A substantial advantage of the new automated approach is high sample throughput thanks to the analysis time of 7.5min, miniaturization via down-scaling the extraction column, and smaller sample and solvent consumption, as well as reduced generation of waste.

Název v anglickém jazyce

Fully automated method based on on-line molecularly imprinted polymer solid-phase extraction for determination of lovastatin in dietary supplements containing red yeast rice

Popis výsledku anglicky

A fully automated method for the determination of lovastatin in dietary supplements containing red yeast rice has been developed. It uses a sequential injection analysis system combined with solid-phase extraction applying highly selective molecularly imprinted polymer sorbent. A miniaturized column for on-line extraction was prepared by packing 4.5mg of the sorbent in a 5.0x2.5-mm-i.d. cartridge, which was used in the flow manifold. Sequential injection analysis manifold enabled all steps of lovastatin extraction and continuous spectrophotometric detection at 240nm. A limit of detection of 60 mu g g(-1), a limit of quantitation of 200 mu g g(-1), and a linear calibration range of 200-2000 mu g g(-1) were achieved. Intra-day and inter-day precision values (RSD) were &lt;=6.7% and &lt;=4.9%, respectively, and method recovery values of spiked red yeast rice extracts at 200, 1000, and 2000 mu g g(-1) concentration levels were 82.9, 95.2, and 87.7%. Our method was used for determination of lovastatin lactone in four dietary supplements containing red yeast rice as a natural source of lovastatin, also known as monacolin K. The extracted samples were subsequently analyzed by the reference UHPLC-MS/MS method. Statistical comparison of results (F test, t test, alpha=0.05) obtained by both methods did not reveal significant difference. A substantial advantage of the new automated approach is high sample throughput thanks to the analysis time of 7.5min, miniaturization via down-scaling the extraction column, and smaller sample and solvent consumption, as well as reduced generation of waste.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/EF16_019%2F0000841" target="_blank" >EF16_019/0000841: Zvýšení účinnosti a bezpečnosti léčiv a nutraceutik: moderní metody - nové výzvy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

—

Svazek periodika

411

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

1219-1228

Kód UT WoS článku

000458358400010

EID výsledku v databázi Scopus

2-s2.0-85059704439