Novel application of capillary electrophoresis with a liposome coated capillary for prediction of blood-brain barrier permeability
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F20%3A10417669" target="_blank" >RIV/00216208:11160/20:10417669 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00179906:_____/20:10417669
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=y4OW8IJ7R4" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=y4OW8IJ7R4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2020.121023" target="_blank" >10.1016/j.talanta.2020.121023</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Novel application of capillary electrophoresis with a liposome coated capillary for prediction of blood-brain barrier permeability
Popis výsledku v původním jazyce
Profiling blood-brain barrier permeability of bioactive molecule is an important issue in early drug development, being a part of the optimization process of a compound's physicochemical properties, and hence pharmacokinetic profile. The study aimed to develop and optimize a new in vitro method for assessment of the compound's brain penetration. The tool is proposed as an alternative to the PAMPA-BBB (Parallel Artificial Membrane Permeability Assay for Blood-Brain Barrier) and based on a capillary electrochromatography (CEC) technique. It utilizes liposomes as structural substitutes of biological membranes, which are used as a capillary inner wall coating material. Following optimization of analysis conditions, migration times for a set of 25 reference drugs (mainly non-ionized in pH 7.4) were examined in a liposome coated capillary. On that basis, the retention factor (log k) was determined for each reference drug. Obtained log k values and experimentally received reference permeability parameters: log BB (in vivo data) and log P-e (PAMPA-BBB data) were compared with one another. Correlation coefficients were calculated, giving comparable results for CEC log k/log BB and analogical PAMPA-BBB log P-e/log BB analyses. Approximate ranges of log k for the central nervous system (CNS) permeable (CNS (+)) and non-permeable (CNS(-)) drugs were established.
Název v anglickém jazyce
Novel application of capillary electrophoresis with a liposome coated capillary for prediction of blood-brain barrier permeability
Popis výsledku anglicky
Profiling blood-brain barrier permeability of bioactive molecule is an important issue in early drug development, being a part of the optimization process of a compound's physicochemical properties, and hence pharmacokinetic profile. The study aimed to develop and optimize a new in vitro method for assessment of the compound's brain penetration. The tool is proposed as an alternative to the PAMPA-BBB (Parallel Artificial Membrane Permeability Assay for Blood-Brain Barrier) and based on a capillary electrochromatography (CEC) technique. It utilizes liposomes as structural substitutes of biological membranes, which are used as a capillary inner wall coating material. Following optimization of analysis conditions, migration times for a set of 25 reference drugs (mainly non-ionized in pH 7.4) were examined in a liposome coated capillary. On that basis, the retention factor (log k) was determined for each reference drug. Obtained log k values and experimentally received reference permeability parameters: log BB (in vivo data) and log P-e (PAMPA-BBB data) were compared with one another. Correlation coefficients were calculated, giving comparable results for CEC log k/log BB and analogical PAMPA-BBB log P-e/log BB analyses. Approximate ranges of log k for the central nervous system (CNS) permeable (CNS (+)) and non-permeable (CNS(-)) drugs were established.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30104 - Pharmacology and pharmacy
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Talanta
ISSN
0039-9140
e-ISSN
—
Svazek periodika
217
Číslo periodika v rámci svazku
SEP
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
14
Strana od-do
121023
Kód UT WoS článku
000537880200025
EID výsledku v databázi Scopus
2-s2.0-85083031224