Determination of the spectrum of low molecular mass organic acids in urine by capillary electrophoresis with contactless conductivity and ultraviolet photometric detection-An efficient tool for monitoring of inborn metabolic disorders

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10104480" target="_blank" >RIV/00216208:11310/11:10104480 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/11:00002418

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.aca.2010.11.007" target="_blank" >http://dx.doi.org/10.1016/j.aca.2010.11.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2010.11.007" target="_blank" >10.1016/j.aca.2010.11.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of the spectrum of low molecular mass organic acids in urine by capillary electrophoresis with contactless conductivity and ultraviolet photometric detection-An efficient tool for monitoring of inborn metabolic disorders

Popis výsledku v původním jazyce

A mixture of 29 organic acids (OAs) occurring in urine was analyzed by capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C4D) and UV photometric detection. The optimized analytical system involved a 100 cm longpolyacrylamide-coated capillary (50 ?m i.d.) and the background electrolyte of 20 mM 2-morpholinoethanesulfonic acid (MES)/NaOH + 10% (v/v) methanol, pH 6.0 (pH is related to the 20 mM MES/NaOH buffer in water). The LOD values obtained by C4D for the OAswhich do not absorb UV radiation range from 0.6 ?M (oxalic acid) to 6.8 ?M (pyruvic acid); those obtained by UV photometry for the remaining OAs range from 2.9 ?M (5-hydroxy-3-indoleacetic acid) to 10.2 ?M (uric acid). The repeatability of the proceduredeveloped is characterized by the coefficients of variation, which vary between 0.3% (tartaric acid) and 0.6% (5-hydroxy-3-indoleacetic acid) for the migration time and between 1.3% (tartaric acid) and 3.5% (lactic acid) for the peak are

Název v anglickém jazyce

Determination of the spectrum of low molecular mass organic acids in urine by capillary electrophoresis with contactless conductivity and ultraviolet photometric detection-An efficient tool for monitoring of inborn metabolic disorders

Popis výsledku anglicky

A mixture of 29 organic acids (OAs) occurring in urine was analyzed by capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C4D) and UV photometric detection. The optimized analytical system involved a 100 cm longpolyacrylamide-coated capillary (50 ?m i.d.) and the background electrolyte of 20 mM 2-morpholinoethanesulfonic acid (MES)/NaOH + 10% (v/v) methanol, pH 6.0 (pH is related to the 20 mM MES/NaOH buffer in water). The LOD values obtained by C4D for the OAswhich do not absorb UV radiation range from 0.6 ?M (oxalic acid) to 6.8 ?M (pyruvic acid); those obtained by UV photometry for the remaining OAs range from 2.9 ?M (5-hydroxy-3-indoleacetic acid) to 10.2 ?M (uric acid). The repeatability of the proceduredeveloped is characterized by the coefficients of variation, which vary between 0.3% (tartaric acid) and 0.6% (5-hydroxy-3-indoleacetic acid) for the migration time and between 1.3% (tartaric acid) and 3.5% (lactic acid) for the peak are

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GAP206%2F10%2F1231" target="_blank" >GAP206/10/1231: Nové elekroforetické přístupy k monitorování hladin neurotransmiterů bolesti v mozku vyšších živočichů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

—

Svazek periodika

685

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

84-90

Kód UT WoS článku

000286856100012

EID výsledku v databázi Scopus

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