Stability of influenza virus as evaluated by integrity of its RNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10125432" target="_blank" >RIV/00216208:11310/12:10125432 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.4149/av_2012_02_125" target="_blank" >http://dx.doi.org/10.4149/av_2012_02_125</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/av_2012_02_125" target="_blank" >10.4149/av_2012_02_125</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Stability of influenza virus as evaluated by integrity of its RNA

Popis výsledku v původním jazyce

Various methods of handling samples of avian influenza prior to detecting influenza viruses can significantly influence both, the detection of the virus and the quantification of viral nucleic acids. The quantity of influenza viral RNA remaining in different collecting buffers and kept at temperatures of -20 degrees C, +4 degrees C or +22 degrees C for various lengths of time, was determined. The quantity of viral RNA remained the same for 120 days at -20 degrees C, but decreased when the samples were stored at either +4 degrees C or +22 degrees C. The quantity of RNA was influenced by the composition of the collecting buffer. The influenza virus sample that is to be used for RNA quantification can be stored at +4 degrees C and freeze and thaw cycles should be avoided during transport. Our results clearly indicate that the quality and quantity of influenza virus nucleic acid depends on the chemical composition of used buffer and also that the samples can be protected from degradation e

Název v anglickém jazyce

Stability of influenza virus as evaluated by integrity of its RNA

Popis výsledku anglicky

Various methods of handling samples of avian influenza prior to detecting influenza viruses can significantly influence both, the detection of the virus and the quantification of viral nucleic acids. The quantity of influenza viral RNA remaining in different collecting buffers and kept at temperatures of -20 degrees C, +4 degrees C or +22 degrees C for various lengths of time, was determined. The quantity of viral RNA remained the same for 120 days at -20 degrees C, but decreased when the samples were stored at either +4 degrees C or +22 degrees C. The quantity of RNA was influenced by the composition of the collecting buffer. The influenza virus sample that is to be used for RNA quantification can be stored at +4 degrees C and freeze and thaw cycles should be avoided during transport. Our results clearly indicate that the quality and quantity of influenza virus nucleic acid depends on the chemical composition of used buffer and also that the samples can be protected from degradation e

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/SP%2F2D3%2F60%2F08" target="_blank" >SP/2D3/60/08: Integrovaný výzkum vlivů a důsledků vypouštění uměle odchovaných kachen divokých na vodní a mokřadní ekosystémy s použitím moderních ekologických a genetických přístupů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Virologica

ISSN

0001-723X

e-ISSN

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Svazek periodika

56

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

4

Strana od-do

125-128

Kód UT WoS článku

000307860400005

EID výsledku v databázi Scopus

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