Multilocus Microsatellite Typing (MLMT) of Strains from Turkey and Cyprus Reveals a Novel Monophyletic L. donovani Sensu Lato Group
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10127498" target="_blank" >RIV/00216208:11310/12:10127498 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1371/journal.pntd.0001507" target="_blank" >http://dx.doi.org/10.1371/journal.pntd.0001507</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pntd.0001507" target="_blank" >10.1371/journal.pntd.0001507</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Multilocus Microsatellite Typing (MLMT) of Strains from Turkey and Cyprus Reveals a Novel Monophyletic L. donovani Sensu Lato Group
Popis výsledku v původním jazyce
New foci of human CL caused by strains of the Leishmania donovani (L. donovani) complex have been recently described in Cyprus and the C, ukurova region in Turkey (L. infantum) situated 150 km north of Cyprus. Cypriot strains were typed by Multilocus Enzyme Electrophoresis (MLEE) using the Montpellier (MON) system as L. donovani zymodeme MON-37. However, multilocus microsatellite typing (MLMT) has shown that this zymodeme is paraphyletic; composed of distantly related genetic subgroups of different geographical origin. Methodology/Principal Findings: The Cypriot strains were compared with a set of Turkish isolates obtained from a CL patient and sand fly vectors in south-east Turkey (C, ukurova region; CUK strains) and from a VL patient in the south-west (Kusadasi; EP59 strain). These Turkish strains were initially analyzed using the K26-PCR assay that discriminates MON-1 strains by their amplicon size. In line with previous DNA-based data, the strains were inferred to the L. donovani c
Název v anglickém jazyce
Multilocus Microsatellite Typing (MLMT) of Strains from Turkey and Cyprus Reveals a Novel Monophyletic L. donovani Sensu Lato Group
Popis výsledku anglicky
New foci of human CL caused by strains of the Leishmania donovani (L. donovani) complex have been recently described in Cyprus and the C, ukurova region in Turkey (L. infantum) situated 150 km north of Cyprus. Cypriot strains were typed by Multilocus Enzyme Electrophoresis (MLEE) using the Montpellier (MON) system as L. donovani zymodeme MON-37. However, multilocus microsatellite typing (MLMT) has shown that this zymodeme is paraphyletic; composed of distantly related genetic subgroups of different geographical origin. Methodology/Principal Findings: The Cypriot strains were compared with a set of Turkish isolates obtained from a CL patient and sand fly vectors in south-east Turkey (C, ukurova region; CUK strains) and from a VL patient in the south-west (Kusadasi; EP59 strain). These Turkish strains were initially analyzed using the K26-PCR assay that discriminates MON-1 strains by their amplicon size. In line with previous DNA-based data, the strains were inferred to the L. donovani c
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EG - Zoologie
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/LC06009" target="_blank" >LC06009: Centrum molekulární ekologie vektorů a patogenů</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
PLoS Neglected Tropical Diseases
ISSN
1935-2735
e-ISSN
—
Svazek periodika
6
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
12
Strana od-do
—
Kód UT WoS článku
000300949200019
EID výsledku v databázi Scopus
2-s2.0-84857866348