Genome wide identification of promoter binding sites for H4K12ac in human sperm and its relevance for early embryonic development

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10128734" target="_blank" >RIV/00216208:11310/12:10128734 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.4161/epi.21556" target="_blank" >http://dx.doi.org/10.4161/epi.21556</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4161/epi.21556" target="_blank" >10.4161/epi.21556</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genome wide identification of promoter binding sites for H4K12ac in human sperm and its relevance for early embryonic development

Popis výsledku v původním jazyce

Sperm chromatin reveals two characteristic features in that protamines are the predominant nuclear proteins and remaining histones are highly acetylated. Histone H4 acetylated at lysine 12 (H4K12ac) is localized in the post-acrosomal region, while protamine-1 is present within the whole nucleus. Chromatin immunoprecipitation in combination with promoter array analysis allowed genome-wide identification of H4K12ac binding sites. Previously, we reported enrichment of H4K12ac at CTCF binding sites and promoters of genes involved in developmental processes. Here, we demonstrate that H4K12ac is enriched predominantly between +/- 2 kb from the transcription start site. In addition, we identified developmentally relevant H4K12ac-associated promoters with highexpression levels of their transcripts stored in mature sperm. The highest expressed mRNA codes for testis-specific PHD finger protein-7 (PHF7), suggesting an activating role of H4K12ac in the regulatory elements of this gene. H4K12ac-as

Název v anglickém jazyce

Genome wide identification of promoter binding sites for H4K12ac in human sperm and its relevance for early embryonic development

Popis výsledku anglicky

Sperm chromatin reveals two characteristic features in that protamines are the predominant nuclear proteins and remaining histones are highly acetylated. Histone H4 acetylated at lysine 12 (H4K12ac) is localized in the post-acrosomal region, while protamine-1 is present within the whole nucleus. Chromatin immunoprecipitation in combination with promoter array analysis allowed genome-wide identification of H4K12ac binding sites. Previously, we reported enrichment of H4K12ac at CTCF binding sites and promoters of genes involved in developmental processes. Here, we demonstrate that H4K12ac is enriched predominantly between +/- 2 kb from the transcription start site. In addition, we identified developmentally relevant H4K12ac-associated promoters with highexpression levels of their transcripts stored in mature sperm. The highest expressed mRNA codes for testis-specific PHD finger protein-7 (PHF7), suggesting an activating role of H4K12ac in the regulatory elements of this gene. H4K12ac-as

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP506%2F12%2F1046" target="_blank" >GAP506/12/1046: Evoluce chemické komunikace savců a ptáků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Epigenetics : official journal of the DNA Methylation Society

ISSN

1559-2294

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

1057-1070

Kód UT WoS článku

000308693600011

EID výsledku v databázi Scopus

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