Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10294696" target="_blank" >RIV/00216208:11310/15:10294696 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/15:00443675

Výsledek na webu

<a href="http://dx.doi.org/10.1186/s13148-015-0058-4" target="_blank" >http://dx.doi.org/10.1186/s13148-015-0058-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13148-015-0058-4" target="_blank" >10.1186/s13148-015-0058-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients

Popis výsledku v původním jazyce

Histone to protamine exchange and the hyperacetylation of the remaining histones are hallmarks of spermiogenesis. Acetylation of histone H4 at lysine 12 (H4K12ac) was observed prior to full decondensation of sperm chromatin after fertilization suggestingan important role for the regulation of gene expression in early embryogenesis. Similarly, DNA methylation may contribute to gene silencing of several developmentally important genes. Following the identification of H4K12ac-binding promoters in sperm offertile and subfertile patients, we aimed to investigate whether the depletion of histone-binding is associated with aberrant DNA methylation in sperm of subfertile men. Furthermore, we monitored the transmission of H4K12ac, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) from the paternal chromatin to the embryo applying mouse in vitro fertilization and immunofluorescence. We found 5% to 10% hypomethylation within CpG islands of selected promoters in the sperm of fertile dono

Název v anglickém jazyce

Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients

Popis výsledku anglicky

Histone to protamine exchange and the hyperacetylation of the remaining histones are hallmarks of spermiogenesis. Acetylation of histone H4 at lysine 12 (H4K12ac) was observed prior to full decondensation of sperm chromatin after fertilization suggestingan important role for the regulation of gene expression in early embryogenesis. Similarly, DNA methylation may contribute to gene silencing of several developmentally important genes. Following the identification of H4K12ac-binding promoters in sperm offertile and subfertile patients, we aimed to investigate whether the depletion of histone-binding is associated with aberrant DNA methylation in sperm of subfertile men. Furthermore, we monitored the transmission of H4K12ac, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) from the paternal chromatin to the embryo applying mouse in vitro fertilization and immunofluorescence. We found 5% to 10% hypomethylation within CpG islands of selected promoters in the sperm of fertile dono

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinical Epigenetics [online]

ISSN

1868-7083

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

MAR 19 2015

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

17

Strana od-do

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Kód UT WoS článku

000351573300001

EID výsledku v databázi Scopus

2-s2.0-84927154461