Efficient high-throughput sequencing of a laser microdissected chromosome arm

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10189110" target="_blank" >RIV/00216208:11310/13:10189110 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/13:#0001029

Výsledek na webu

<a href="http://dx.doi.org/10.1186/1471-2164-14-357" target="_blank" >http://dx.doi.org/10.1186/1471-2164-14-357</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1471-2164-14-357" target="_blank" >10.1186/1471-2164-14-357</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Efficient high-throughput sequencing of a laser microdissected chromosome arm

Popis výsledku v původním jazyce

Background: Genomic sequence assemblies are key tools for a broad range of gene function and evolutionary studies. The diploid amphibian Xenopus tropicalis plays a pivotal role in these fields due to its combination of experimental flexibility, diploid genome, and early-branching tetrapod taxonomic position, having diverged from the amniote lineage similar to 360 million years ago. A genome assembly and a genetic linkage map have recently been made available. Unfortunately, large gaps in the linkage mapattenuate long-range integrity of the genome assembly. Results: We laser dissected the short arm of X. tropicalis chromosome 7 for next generation sequencing and computational mapping to the reference genome. This arm is of particular interest as it encodes the sex determination locus, but its genetic map contains large gaps which undermine available genome assemblies. Whole genome amplification of 15 laser-microdissected 7p arms followed by next generation sequencing yielded similar to

Název v anglickém jazyce

Efficient high-throughput sequencing of a laser microdissected chromosome arm

Popis výsledku anglicky

Background: Genomic sequence assemblies are key tools for a broad range of gene function and evolutionary studies. The diploid amphibian Xenopus tropicalis plays a pivotal role in these fields due to its combination of experimental flexibility, diploid genome, and early-branching tetrapod taxonomic position, having diverged from the amniote lineage similar to 360 million years ago. A genome assembly and a genetic linkage map have recently been made available. Unfortunately, large gaps in the linkage mapattenuate long-range integrity of the genome assembly. Results: We laser dissected the short arm of X. tropicalis chromosome 7 for next generation sequencing and computational mapping to the reference genome. This arm is of particular interest as it encodes the sex determination locus, but its genetic map contains large gaps which undermine available genome assemblies. Whole genome amplification of 15 laser-microdissected 7p arms followed by next generation sequencing yielded similar to

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GPP502%2F11%2FP522" target="_blank" >GPP502/11/P522: Fyzické mapování genomu Xenopus tropicalis.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC Genomics

ISSN

1471-2164

e-ISSN

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Svazek periodika

14

Číslo periodika v rámci svazku

May 2013

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000321385600001

EID výsledku v databázi Scopus

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