The choice of reference gene set for assessing gene expression in barley (Hordeum vulgare L.) under low temperature and drought stress

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10195352" target="_blank" >RIV/00216208:11310/13:10195352 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027006:_____/13:00002634

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00438-013-0774-4" target="_blank" >http://dx.doi.org/10.1007/s00438-013-0774-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00438-013-0774-4" target="_blank" >10.1007/s00438-013-0774-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The choice of reference gene set for assessing gene expression in barley (Hordeum vulgare L.) under low temperature and drought stress

Popis výsledku v původním jazyce

Drought and low temperature are the two most significant causes of abiotic stress in agricultural crops and, therefore, they pose considerable challenges in plant science. Hence, it is crucial to study response mechanisms and to select genes for identification signaling pathways that lead from stimulus to response. The assessment of gene expression is often attempted using real-time RT-PCR (qRT-PCR), a technique which requires a careful choice of reference gene(s) for normalization purpose. Here, we report a comparison of 13 potential reference genes for studying gene expression in the leaf and crown of barley seedlings subjected to low temperature or drought stress. All three currently available software packages designed to identify reference genes from qRT-PCR data (GeNorm, NormFinder and BestKeeper) were used to identify informative sets of up to three reference genes. Interestingly, the data obtained from the separate treatment of leaf and crown have led to the recommendations tha

Název v anglickém jazyce

The choice of reference gene set for assessing gene expression in barley (Hordeum vulgare L.) under low temperature and drought stress

Popis výsledku anglicky

Drought and low temperature are the two most significant causes of abiotic stress in agricultural crops and, therefore, they pose considerable challenges in plant science. Hence, it is crucial to study response mechanisms and to select genes for identification signaling pathways that lead from stimulus to response. The assessment of gene expression is often attempted using real-time RT-PCR (qRT-PCR), a technique which requires a careful choice of reference gene(s) for normalization purpose. Here, we report a comparison of 13 potential reference genes for studying gene expression in the leaf and crown of barley seedlings subjected to low temperature or drought stress. All three currently available software packages designed to identify reference genes from qRT-PCR data (GeNorm, NormFinder and BestKeeper) were used to identify informative sets of up to three reference genes. Interestingly, the data obtained from the separate treatment of leaf and crown have led to the recommendations tha

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Genetics and Genomics

ISSN

1617-4615

e-ISSN

—

Svazek periodika

288

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

639-649

Kód UT WoS článku

000326263900008

EID výsledku v databázi Scopus

—