Identification of the most suitable reference gene for gene expression studies with development and abiotic stress response in Bromus sterilis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F21%3A88334" target="_blank" >RIV/60460709:41210/21:88334 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41320/21:88334

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-021-92780-1#Abs1" target="_blank" >https://www.nature.com/articles/s41598-021-92780-1#Abs1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-021-92780-1" target="_blank" >10.1038/s41598-021-92780-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of the most suitable reference gene for gene expression studies with development and abiotic stress response in Bromus sterilis

Popis výsledku v původním jazyce

Bromus sterilis is an annual weedy grass, causing high yield losses in winter cereals. Frequent use of herbicides had led to the evolution of herbicide resistance in this species. Mechanisms underlying herbicide resistance in B. sterilis must be uncovered because this problem is becoming a global threat. qRT-PCR and the next-generation sequencing technologies can elucidate the resistance mechanisms. Although qRT-PCR can calculate precise fold changes, its preciseness depends on the expression of reference genes. Regardless of stable expression in any given condition, no gene can act as a universal reference gene. Hence, it is necessary to identify the suitable reference gene for each species. To our knowledge, there are no reports on the suitable reference gene in any brome species so far. Thus, in this paper, the stability of eight genes was evaluated using qRT-PCR experiments followed by expression stability ranking via five most commonly used software for reference gene selection. Our findings sug

Název v anglickém jazyce

Identification of the most suitable reference gene for gene expression studies with development and abiotic stress response in Bromus sterilis

Popis výsledku anglicky

Bromus sterilis is an annual weedy grass, causing high yield losses in winter cereals. Frequent use of herbicides had led to the evolution of herbicide resistance in this species. Mechanisms underlying herbicide resistance in B. sterilis must be uncovered because this problem is becoming a global threat. qRT-PCR and the next-generation sequencing technologies can elucidate the resistance mechanisms. Although qRT-PCR can calculate precise fold changes, its preciseness depends on the expression of reference genes. Regardless of stable expression in any given condition, no gene can act as a universal reference gene. Hence, it is necessary to identify the suitable reference gene for each species. To our knowledge, there are no reports on the suitable reference gene in any brome species so far. Thus, in this paper, the stability of eight genes was evaluated using qRT-PCR experiments followed by expression stability ranking via five most commonly used software for reference gene selection. Our findings sug

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1820081" target="_blank" >QK1820081: Metody monitorovaní rezistence hospodářsky významných škůdců a plevelů k přípravkům na ochranu rostlin a antirezistentní strategie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

0-0

Kód UT WoS článku

000669974100006

EID výsledku v databázi Scopus

2-s2.0-85108825029