DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10210464" target="_blank" >RIV/00216208:11310/14:10210464 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.2478/s11532-014-0516-4" target="_blank" >http://dx.doi.org/10.2478/s11532-014-0516-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/s11532-014-0516-4" target="_blank" >10.2478/s11532-014-0516-4</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea
Popis výsledku v původním jazyce
Novel electrochemical DNA-based biosensors with outer-sphere Nafion and chitosan protective membranes were prepared for the evaluation of antioxidant properties of beverages (beer, coffee, and black tea) against prooxidant hydroxyl radicals. A carbon working electrode of a screen-printed three-electrode assembly was modified using a layer-by-layer deposition technique with low molecular weight double-stranded DNA and a Nafion or chitosan film. The membrane-covered DNA biosensors were initially tested with respect to their voltammetric and impedimetric response after the incubation of the beverage and the medium exchange for the solution of the redox indicator [Fe(CN)(6)](3-/4-). While the Nafion-protected biosensor proved to be suitable for beer and black tea extracts, the chitosan-protected biosensor was successfully used in a coffee extract. Afterwards, the applicability was successfully verified for these biosensors for the detection of a deep degradation of the surface-attached DNA
Název v anglickém jazyce
DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea
Popis výsledku anglicky
Novel electrochemical DNA-based biosensors with outer-sphere Nafion and chitosan protective membranes were prepared for the evaluation of antioxidant properties of beverages (beer, coffee, and black tea) against prooxidant hydroxyl radicals. A carbon working electrode of a screen-printed three-electrode assembly was modified using a layer-by-layer deposition technique with low molecular weight double-stranded DNA and a Nafion or chitosan film. The membrane-covered DNA biosensors were initially tested with respect to their voltammetric and impedimetric response after the incubation of the beverage and the medium exchange for the solution of the redox indicator [Fe(CN)(6)](3-/4-). While the Nafion-protected biosensor proved to be suitable for beer and black tea extracts, the chitosan-protected biosensor was successfully used in a coffee extract. Afterwards, the applicability was successfully verified for these biosensors for the detection of a deep degradation of the surface-attached DNA
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CG - Elektrochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GP13-23337P" target="_blank" >GP13-23337P: Komplexní diagnostický přístup při hodnocení interakce DNA s organickými xenobiotiky pomocí elektrochemických nanostrukturovaných DNA biosenzorů</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Central European Journal of Chemistry
ISSN
1895-1066
e-ISSN
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Svazek periodika
12
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
PL - Polská republika
Počet stran výsledku
8
Strana od-do
604-611
Kód UT WoS článku
000331644400008
EID výsledku v databázi Scopus
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