Expression Levels of Enzymes Metabolizing an Anticancer Drug Ellipticine Determined by Electromigration Assays Influence its Cytotoxicity to Cancer Cells - A Comparative Study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10282893" target="_blank" >RIV/00216208:11310/14:10282893 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/14:00225107 RIV/00216208:11130/14:10282893 RIV/00216305:26620/14:PU112646 RIV/00064203:_____/14:10282893

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol9/91005675.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol9/91005675.pdf</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Expression Levels of Enzymes Metabolizing an Anticancer Drug Ellipticine Determined by Electromigration Assays Influence its Cytotoxicity to Cancer Cells - A Comparative Study

Popis výsledku v původním jazyce

The antineoplastic alkaloid ellipticine is a prodrug, of which the pharmacological efficiency is dependent on its cytochrome P450 (CYP) - and/or peroxidase-mediated activation to 12-hydroxy- and 13-hydroxyellipticine, which are both the metabolites forming DNA adducts in target tissues. Using the method of Western blotting, the expression of CYP1A1, 1B1, 3A4, lactoperoxidase, thyroid peroxidase, cyclooxygenase-1 and cytochrome b5, the enzymes that catalyze and/or influence ellipticine metabolism, was investigated in several cancer cells sensitive to ellipticine (HL-60 promyelocytic leukemia and T-cell leukemia CCRF-CEM cells, glioblastoma U87MG cells, thyroid cancer BHT-101, B-CPAP and 8505-C cells, neuroblastoma UKF-NB-3 and UKF-NB-4 cell lines and breast adenocarcinoma MCF-7 cells). The findings summarized from several former studies reviewed in this study, together with new results indicate that, depending on individual cells, cytotoxicity of ellipticine, which is mediated by format

Název v anglickém jazyce

Expression Levels of Enzymes Metabolizing an Anticancer Drug Ellipticine Determined by Electromigration Assays Influence its Cytotoxicity to Cancer Cells - A Comparative Study

Popis výsledku anglicky

The antineoplastic alkaloid ellipticine is a prodrug, of which the pharmacological efficiency is dependent on its cytochrome P450 (CYP) - and/or peroxidase-mediated activation to 12-hydroxy- and 13-hydroxyellipticine, which are both the metabolites forming DNA adducts in target tissues. Using the method of Western blotting, the expression of CYP1A1, 1B1, 3A4, lactoperoxidase, thyroid peroxidase, cyclooxygenase-1 and cytochrome b5, the enzymes that catalyze and/or influence ellipticine metabolism, was investigated in several cancer cells sensitive to ellipticine (HL-60 promyelocytic leukemia and T-cell leukemia CCRF-CEM cells, glioblastoma U87MG cells, thyroid cancer BHT-101, B-CPAP and 8505-C cells, neuroblastoma UKF-NB-3 and UKF-NB-4 cell lines and breast adenocarcinoma MCF-7 cells). The findings summarized from several former studies reviewed in this study, together with new results indicate that, depending on individual cells, cytotoxicity of ellipticine, which is mediated by format

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP301%2F10%2F0356" target="_blank" >GAP301/10/0356: Studie participace specifických mechanismů poškození DNA na cytoxicitě cytostatik vůči lidským chemosensitivním a chemorestentním neuroblastomům</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

15

Strana od-do

5675-5689

Kód UT WoS článku

000345261700024

EID výsledku v databázi Scopus

—