Direct CE and HPLC methods for enantioseparation of tryptophan and its unnatural derivatives

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10325321" target="_blank" >RIV/00216208:11310/16:10325321 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.seppur.2015.12.012" target="_blank" >http://dx.doi.org/10.1016/j.seppur.2015.12.012</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.seppur.2015.12.012" target="_blank" >10.1016/j.seppur.2015.12.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Direct CE and HPLC methods for enantioseparation of tryptophan and its unnatural derivatives

Popis výsledku v původním jazyce

Tryptophan and its eight derivatives considered in this work are biologically important compounds. Since their enantiomers can exhibit different behavior, efficient enantioselective separation methods are needed for both analytical and preparative purposes. In capillary electrophoresis cyclodextrins and their derivatives were proved to be suitable chiral selectors. Two pH values of background electrolytes were tested in order to affect ionization of the analytes and consequently their enantioseparation. Enantiomers of all analytes in this study were baseline separated within 8 min using capillary electrophoresis. However, different separation systems/conditions were required. In HPLC various separation modes and columns (based on derivatized polysaccharides, cyclofructan, cyclodextrin and teicoplanin) were used. The best results of enantioseparation of tryptophan and its amphoteric derivatives were achieved with teicoplanin based chiral stationary phases and methanol as a mobile phase. Proposed conditions were suitable even for purification purposes. This study can serve as a tool for simplifying the method development for enantioseparation of tryptophan and its derivatives.

Název v anglickém jazyce

Direct CE and HPLC methods for enantioseparation of tryptophan and its unnatural derivatives

Popis výsledku anglicky

Tryptophan and its eight derivatives considered in this work are biologically important compounds. Since their enantiomers can exhibit different behavior, efficient enantioselective separation methods are needed for both analytical and preparative purposes. In capillary electrophoresis cyclodextrins and their derivatives were proved to be suitable chiral selectors. Two pH values of background electrolytes were tested in order to affect ionization of the analytes and consequently their enantioseparation. Enantiomers of all analytes in this study were baseline separated within 8 min using capillary electrophoresis. However, different separation systems/conditions were required. In HPLC various separation modes and columns (based on derivatized polysaccharides, cyclofructan, cyclodextrin and teicoplanin) were used. The best results of enantioseparation of tryptophan and its amphoteric derivatives were achieved with teicoplanin based chiral stationary phases and methanol as a mobile phase. Proposed conditions were suitable even for purification purposes. This study can serve as a tool for simplifying the method development for enantioseparation of tryptophan and its derivatives.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GP14-19278P" target="_blank" >GP14-19278P: Komplexace analytů a složek pufru s komplexujícím činidlem probíhající současně v analytických separačních systémech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Separation and Purification Technology

ISSN

1383-5866

e-ISSN

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Svazek periodika

158

Číslo periodika v rámci svazku

158

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

24-30

Kód UT WoS článku

000369461700004

EID výsledku v databázi Scopus

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