Direct injection mass spectrometry, thin layer chromatography, and gas chromatography of Bacillus subtilis phospholipids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10325666" target="_blank" >RIV/00216208:11310/16:10325666 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064165:_____/16:10325666

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00706-016-1734-6" target="_blank" >http://dx.doi.org/10.1007/s00706-016-1734-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-016-1734-6" target="_blank" >10.1007/s00706-016-1734-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Direct injection mass spectrometry, thin layer chromatography, and gas chromatography of Bacillus subtilis phospholipids

Popis výsledku v původním jazyce

Direct injection mass spectrometry was amended with chromatographic methods in analysis of membrane phospholipids of Bacillus subtilis mutant strain, which contains four polar headgroups only (phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, and phosphatidic acid). Phospholipid classes were determined by thin layer chromatography as the basic method. Analysis of fatty acids by gas chromatography determined relative abundances of saturated, unsaturated, linear, and branched C-14 to C-18 fatty acids in phospholipids. Experimental data measured by direct injection mass spectrometry in the m/z range of 590-790 were used to estimate theoretical phospholipid composition which was compared with the data of chromatographic methods. The phospholipid class relative abundances measured by mass spectrometry did not agree fully with those of thin layer chromatography and the probable explanation was proposed. The direct injection mass spectrometry approach seems to be fast and easy procedure suitable for analyses of proportional changes in phospholipid composition and can be used as a complementary method for trend analyses and in process monitoring.

Název v anglickém jazyce

Direct injection mass spectrometry, thin layer chromatography, and gas chromatography of Bacillus subtilis phospholipids

Popis výsledku anglicky

Direct injection mass spectrometry was amended with chromatographic methods in analysis of membrane phospholipids of Bacillus subtilis mutant strain, which contains four polar headgroups only (phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, and phosphatidic acid). Phospholipid classes were determined by thin layer chromatography as the basic method. Analysis of fatty acids by gas chromatography determined relative abundances of saturated, unsaturated, linear, and branched C-14 to C-18 fatty acids in phospholipids. Experimental data measured by direct injection mass spectrometry in the m/z range of 590-790 were used to estimate theoretical phospholipid composition which was compared with the data of chromatographic methods. The phospholipid class relative abundances measured by mass spectrometry did not agree fully with those of thin layer chromatography and the probable explanation was proposed. The direct injection mass spectrometry approach seems to be fast and easy procedure suitable for analyses of proportional changes in phospholipid composition and can be used as a complementary method for trend analyses and in process monitoring.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/GP13-18051P" target="_blank" >GP13-18051P: Rezistence k surfaktinu u producenta Bacillus subtilis: Molekulární mechanizmus rezistence lipidové složky cytoplazmatické membrány.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie

ISSN

0026-9247

e-ISSN

—

Svazek periodika

147

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

7

Strana od-do

1385-1391

Kód UT WoS článku

000380695200009

EID výsledku v databázi Scopus

2-s2.0-84962249680