Sulfobutylether-beta-cyclodextrin as a chiral selector for separation of amino acids and dipeptides in chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10332108" target="_blank" >RIV/00216208:11310/16:10332108 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2016.07.061" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2016.07.061</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2016.07.061" target="_blank" >10.1016/j.chroma.2016.07.061</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sulfobutylether-beta-cyclodextrin as a chiral selector for separation of amino acids and dipeptides in chromatography

Popis výsledku v původním jazyce

Various amino acids, dipeptides and their isomers were (enantio)separated using sulfobutylether-beta-cyclodextrin as a chiral selector. Two different approaches were employed: first, dynamic coating of sulfobutylether-beta-cyclodextrin onto a strong anion-exchange stationary phase and, second, use of sulfobutylether-beta-cyclodextrin as a mobile phase additive in a separation system with a C18 column. Measurements were carried out using RP-HPLC and hydrophilic interaction liquid chromatography. Mobile phases composed of organic modifier (methanol) and four different aqueous parts: (i) deionized water, (ii) an aqueous solution of formic acid (pH 2.1), (iii) ammonium acetate buffer(pH 4.7), and (iv) ammonium acetate buffer (pH 8.8) in various volume ratios. Under these separation conditions, out of 23 chiral analytes, 9 were baseline enantio-resolved and 7 were partially separated. Of 9 mixtures of dipeptide isomers, 8 were baseline-separated. Sulfobutylether-beta-cyclodextrin proved to be suitable for the separation of chiral and also achiral analytes. The use of sulfobutylether-beta-cyclodextrin as a dynamic coating agent or as a mobile phase additive depends on the particular chromatographic system and analytes of interest.

Název v anglickém jazyce

Sulfobutylether-beta-cyclodextrin as a chiral selector for separation of amino acids and dipeptides in chromatography

Popis výsledku anglicky

Various amino acids, dipeptides and their isomers were (enantio)separated using sulfobutylether-beta-cyclodextrin as a chiral selector. Two different approaches were employed: first, dynamic coating of sulfobutylether-beta-cyclodextrin onto a strong anion-exchange stationary phase and, second, use of sulfobutylether-beta-cyclodextrin as a mobile phase additive in a separation system with a C18 column. Measurements were carried out using RP-HPLC and hydrophilic interaction liquid chromatography. Mobile phases composed of organic modifier (methanol) and four different aqueous parts: (i) deionized water, (ii) an aqueous solution of formic acid (pH 2.1), (iii) ammonium acetate buffer(pH 4.7), and (iv) ammonium acetate buffer (pH 8.8) in various volume ratios. Under these separation conditions, out of 23 chiral analytes, 9 were baseline enantio-resolved and 7 were partially separated. Of 9 mixtures of dipeptide isomers, 8 were baseline-separated. Sulfobutylether-beta-cyclodextrin proved to be suitable for the separation of chiral and also achiral analytes. The use of sulfobutylether-beta-cyclodextrin as a dynamic coating agent or as a mobile phase additive depends on the particular chromatographic system and analytes of interest.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1467

Číslo periodika v rámci svazku

1467

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

356-362

Kód UT WoS článku

000385323800033

EID výsledku v databázi Scopus

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