Folate Receptor beta Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10372961" target="_blank" >RIV/00216208:11310/16:10372961 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.4049/jimmunol.1501878" target="_blank" >http://dx.doi.org/10.4049/jimmunol.1501878</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4049/jimmunol.1501878" target="_blank" >10.4049/jimmunol.1501878</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Folate Receptor beta Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen

Popis výsledku v původním jazyce

Folate, also known as vitamin B-9, is necessary for essential cellular functions such as DNA synthesis, repair, and methylation. It is supplied to the cell via several transporters and receptors, including folate receptor (FR) beta, a GPI-anchored protein belonging to the folate receptor family. As FR beta shows a restricted expression to cells of myeloid origin and only a subset of activated macrophages and placental cells have been shown to express functional FR beta, it represents a promising target for future therapeutic strategies. In this study, we performed affinity purification and mass spectrometric analysis of the protein microenvironment of FR beta in the plasma membrane of human FR beta(+) macrophages and FR beta-transduced monocytic THP-1 cells. In this manner, we identified a novel role of FR beta: that is, we report functional interactions of FR beta with receptors mediating cellular adhesion, in particular the CD11b/CD18 beta(2) integrin heterodimer complement receptor type 3/Mac-1. This interaction results in impeded adhesion of FR beta(+) human primary macrophages and THP-1 cells to collagen in comparison with their FR beta(-) counterparts. We further show that FR beta is only expressed by human macrophages when differentiated with M-CSF. These findings thus identify FR beta as a novel CD11b/CD18 regulator for trafficking and homing of a subset of macrophages on collagen.

Název v anglickém jazyce

Folate Receptor beta Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen

Popis výsledku anglicky

Folate, also known as vitamin B-9, is necessary for essential cellular functions such as DNA synthesis, repair, and methylation. It is supplied to the cell via several transporters and receptors, including folate receptor (FR) beta, a GPI-anchored protein belonging to the folate receptor family. As FR beta shows a restricted expression to cells of myeloid origin and only a subset of activated macrophages and placental cells have been shown to express functional FR beta, it represents a promising target for future therapeutic strategies. In this study, we performed affinity purification and mass spectrometric analysis of the protein microenvironment of FR beta in the plasma membrane of human FR beta(+) macrophages and FR beta-transduced monocytic THP-1 cells. In this manner, we identified a novel role of FR beta: that is, we report functional interactions of FR beta with receptors mediating cellular adhesion, in particular the CD11b/CD18 beta(2) integrin heterodimer complement receptor type 3/Mac-1. This interaction results in impeded adhesion of FR beta(+) human primary macrophages and THP-1 cells to collagen in comparison with their FR beta(-) counterparts. We further show that FR beta is only expressed by human macrophages when differentiated with M-CSF. These findings thus identify FR beta as a novel CD11b/CD18 regulator for trafficking and homing of a subset of macrophages on collagen.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Immunology

ISSN

0022-1767

e-ISSN

—

Svazek periodika

197

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

2229-2238

Kód UT WoS článku

000385003600019

EID výsledku v databázi Scopus

—