Vanillylmandelic and Homovanillic acid: Electroanalysis at non-modified and polymer-modified carbon-based electrodes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10376694" target="_blank" >RIV/00216208:11310/18:10376694 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.jelechem.2018.03.011" target="_blank" >https://doi.org/10.1016/j.jelechem.2018.03.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2018.03.011" target="_blank" >10.1016/j.jelechem.2018.03.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Vanillylmandelic and Homovanillic acid: Electroanalysis at non-modified and polymer-modified carbon-based electrodes

Popis výsledku v původním jazyce

The electrochemical behaviour of two main catecholamine metabolites serving as diagnostic tumour biomarkers, homovanillic acid (HVA) and vanillylmandelic acid (VMA), on anodically oxidized boron doped diamond (BDD) electrodes, non-modified glassy carbon electrodes (GCE), and GCE modified by polymers, namely by Nafion (Nafion/GCE) and by poly(neutral red) (PNR/GCE), was investigated. The voltammetric responses of both biomarkers are strongly dependent on pH of the aqueous buffer solution and electrode material used. BDD electrodes exhibit more positive oxidation potentials and lower current densities for oxidation of both analytes, depending also on the surface termination. Concentration dependences were measured in 0.1 mol L-1 phosphate buffer pH 3.0, as optimal supporting electrolyte for differential pulse voltammetric determination. Achieved detection limits were 0.6, 0.9, 0.8, and 1.2 mu mol L-1 for HVA and 0.4, 1.5, 2.4, and 1.1 mu mol L-1 for VMA at BDD electrodes, non-modified GCE, Nafion/GCE, and PNR/GCE, respectively. The limits of quantification for both organic compounds on all tested electrode materials are sufficiently low for their determination in urine. Verification of the possibility of simultaneous voltammetric determination of the studied biomarkers in the mixture was also carried out.

Název v anglickém jazyce

Vanillylmandelic and Homovanillic acid: Electroanalysis at non-modified and polymer-modified carbon-based electrodes

Popis výsledku anglicky

The electrochemical behaviour of two main catecholamine metabolites serving as diagnostic tumour biomarkers, homovanillic acid (HVA) and vanillylmandelic acid (VMA), on anodically oxidized boron doped diamond (BDD) electrodes, non-modified glassy carbon electrodes (GCE), and GCE modified by polymers, namely by Nafion (Nafion/GCE) and by poly(neutral red) (PNR/GCE), was investigated. The voltammetric responses of both biomarkers are strongly dependent on pH of the aqueous buffer solution and electrode material used. BDD electrodes exhibit more positive oxidation potentials and lower current densities for oxidation of both analytes, depending also on the surface termination. Concentration dependences were measured in 0.1 mol L-1 phosphate buffer pH 3.0, as optimal supporting electrolyte for differential pulse voltammetric determination. Achieved detection limits were 0.6, 0.9, 0.8, and 1.2 mu mol L-1 for HVA and 0.4, 1.5, 2.4, and 1.1 mu mol L-1 for VMA at BDD electrodes, non-modified GCE, Nafion/GCE, and PNR/GCE, respectively. The limits of quantification for both organic compounds on all tested electrode materials are sufficiently low for their determination in urine. Verification of the possibility of simultaneous voltammetric determination of the studied biomarkers in the mixture was also carried out.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA15-02815S" target="_blank" >GA15-02815S: Nové rozpoznávací principy a přístupy k elektrochemické detekci nádorových markerů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

—

Svazek periodika

821

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

22-32

Kód UT WoS článku

000437818600005

EID výsledku v databázi Scopus

2-s2.0-85043496850