Voltammetric determination of homovanillic acid and vanillylmandelic acid on a disposable electrochemical measuring cell system with integrated carbon composite film electrodes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10318274" target="_blank" >RIV/00216208:11310/16:10318274 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00706-015-1583-8" target="_blank" >http://dx.doi.org/10.1007/s00706-015-1583-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-015-1583-8" target="_blank" >10.1007/s00706-015-1583-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Voltammetric determination of homovanillic acid and vanillylmandelic acid on a disposable electrochemical measuring cell system with integrated carbon composite film electrodes

Popis výsledku v původním jazyce

An array of carbon composite electrodes embedded in a 96-well microtitration plate was applied to differential pulse voltammetry determination of standards of tumor biomarkers 2-(4-hydroxy-3-methoxy-phenyl) acetic acid (homovanillic acid, HVA) and (RS)-hydroxy(4-hydroxy-3-methoxy-phenyl) acetic acid (vanillylmandelic acid, VMA). For the preparation of composite electrodes, graphitic conductive microparticles and nonconductive polystyrene binder were used. For the measurement, a buffer of pH 2 was selected as the optimum medium for both analytes. In this medium, concentration dependences of stand-alone standards were measured; calculated quantification limits were 0.3 A mu mol dm(-3) for HVA and 0.8 A mu mol dm(-3) for VMA. Sorption of analytes on employed working electrode was examined in order to verify, whether the current is influenced by the time period between cell filling and measurement. The sorption was not observed and the current was stable even after 10 min. Developed method

Název v anglickém jazyce

Voltammetric determination of homovanillic acid and vanillylmandelic acid on a disposable electrochemical measuring cell system with integrated carbon composite film electrodes

Popis výsledku anglicky

An array of carbon composite electrodes embedded in a 96-well microtitration plate was applied to differential pulse voltammetry determination of standards of tumor biomarkers 2-(4-hydroxy-3-methoxy-phenyl) acetic acid (homovanillic acid, HVA) and (RS)-hydroxy(4-hydroxy-3-methoxy-phenyl) acetic acid (vanillylmandelic acid, VMA). For the preparation of composite electrodes, graphitic conductive microparticles and nonconductive polystyrene binder were used. For the measurement, a buffer of pH 2 was selected as the optimum medium for both analytes. In this medium, concentration dependences of stand-alone standards were measured; calculated quantification limits were 0.3 A mu mol dm(-3) for HVA and 0.8 A mu mol dm(-3) for VMA. Sorption of analytes on employed working electrode was examined in order to verify, whether the current is influenced by the time period between cell filling and measurement. The sorption was not observed and the current was stable even after 10 min. Developed method

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Centrum nových přístupů k bioanalýze a molekulární diagnostice</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie

ISSN

0026-9247

e-ISSN

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Svazek periodika

147

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

8

Strana od-do

89-96

Kód UT WoS článku

000367521400012

EID výsledku v databázi Scopus

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