Behaviour and detection of acridine-type DNA intercalators in urine using an electrochemical DNA-based biosensor with the protective polyvinyl alcohol membrane

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10378555" target="_blank" >RIV/00216208:11310/18:10378555 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.jelechem.2017.11.028" target="_blank" >https://doi.org/10.1016/j.jelechem.2017.11.028</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2017.11.028" target="_blank" >10.1016/j.jelechem.2017.11.028</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Behaviour and detection of acridine-type DNA intercalators in urine using an electrochemical DNA-based biosensor with the protective polyvinyl alcohol membrane

Popis výsledku v původním jazyce

The aim of this work is preparation and use of an electrochemical DNA-based biosensor with a textured surface for the investigation of dsDNA host-guest interactions with specific chemicals in a biological sample of complex matrix such as urine. Results obtained at the search and optimization of polymer protective films over the DNA recognition layer at a glassy carbon electrode show that a polyvinyl alcohol membrane is the effective barrier able to stabilize the biosensor response in urine. The detection of acridines using the square-wave voltammetric (SWV) scan in the cathodic direction has allowed overcoming a problem of the electrochemical interference of DNA bases and acridines at the direct anodic scan. Height of the well-separated SWV peak at around 0.00 V to + 0.10 V (which corresponds to the reduction of the oxidation products of both of acridines) depends on the concentration of acridines. The peak potentials of acridines were shifted by about 0.10 V to the negative potential values in the urine medium compared to phosphate buffer. Intercalation of acridine yellow and proflavine into dsDNA was confirmed both in phosphate buffer and in urine.

Název v anglickém jazyce

Behaviour and detection of acridine-type DNA intercalators in urine using an electrochemical DNA-based biosensor with the protective polyvinyl alcohol membrane

Popis výsledku anglicky

The aim of this work is preparation and use of an electrochemical DNA-based biosensor with a textured surface for the investigation of dsDNA host-guest interactions with specific chemicals in a biological sample of complex matrix such as urine. Results obtained at the search and optimization of polymer protective films over the DNA recognition layer at a glassy carbon electrode show that a polyvinyl alcohol membrane is the effective barrier able to stabilize the biosensor response in urine. The detection of acridines using the square-wave voltammetric (SWV) scan in the cathodic direction has allowed overcoming a problem of the electrochemical interference of DNA bases and acridines at the direct anodic scan. Height of the well-separated SWV peak at around 0.00 V to + 0.10 V (which corresponds to the reduction of the oxidation products of both of acridines) depends on the concentration of acridines. The peak potentials of acridines were shifted by about 0.10 V to the negative potential values in the urine medium compared to phosphate buffer. Intercalation of acridine yellow and proflavine into dsDNA was confirmed both in phosphate buffer and in urine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Centrum nových přístupů k bioanalýze a molekulární diagnostice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

—

Svazek periodika

821

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

5

Strana od-do

87-91

Kód UT WoS článku

000437818600014

EID výsledku v databázi Scopus

2-s2.0-85034849408