Comparison of two pyrolytic graphite representatives in the construction of hybrid electrochemical DNA biosensors for monitoring DNA damage
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10445172" target="_blank" >RIV/00216208:11310/22:10445172 - isvavai.cz</a>
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=S-FOybf46o" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=S-FOybf46o</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jelechem.2022.116095" target="_blank" >10.1016/j.jelechem.2022.116095</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Comparison of two pyrolytic graphite representatives in the construction of hybrid electrochemical DNA biosensors for monitoring DNA damage
Popis výsledku v původním jazyce
Electrochemical DNA biosensors represent a variety of versatile analytical tools successfully used in many important applications. Their utilization in the area of monitoring DNA damage is considered as the prominent field that can provide us with an improved general knowledge regarding DNA damaging events occurring in vivo by an unorthodox overall insight employing the various electrochemical methods. Herein, we present development, optimization process, and subsequent analytical testing of the novel hybrid electrochemical DNA biosensors based on the two representatives of the pyrolytic graphite - "basal-plane" pyrolytic graphite (BPPG) and "edge-plane" pyrolytic graphite (EPPG) - and low-molecular-weight double-stranded DNA (dsDNA). Closer resolution of the nature of the oxidation/reduction signals of the dsDNA, as well as the most important optimization of parameters securing satisfying operational stability of the proposed hybrid biosen-sors, is investigated. Subsequent analytical testing of the novel hybrid biosensors and the evaluation of their advantages/disadvantages in terms of monitoring DNA damage caused by an UV light irradiation employing various voltammetric methods (square wave (SWV), linear sweep (LSV), or cyclic (CV) voltammetry) and elec-trochemical impedance spectroscopy (EIS) is also presented.
Název v anglickém jazyce
Comparison of two pyrolytic graphite representatives in the construction of hybrid electrochemical DNA biosensors for monitoring DNA damage
Popis výsledku anglicky
Electrochemical DNA biosensors represent a variety of versatile analytical tools successfully used in many important applications. Their utilization in the area of monitoring DNA damage is considered as the prominent field that can provide us with an improved general knowledge regarding DNA damaging events occurring in vivo by an unorthodox overall insight employing the various electrochemical methods. Herein, we present development, optimization process, and subsequent analytical testing of the novel hybrid electrochemical DNA biosensors based on the two representatives of the pyrolytic graphite - "basal-plane" pyrolytic graphite (BPPG) and "edge-plane" pyrolytic graphite (EPPG) - and low-molecular-weight double-stranded DNA (dsDNA). Closer resolution of the nature of the oxidation/reduction signals of the dsDNA, as well as the most important optimization of parameters securing satisfying operational stability of the proposed hybrid biosen-sors, is investigated. Subsequent analytical testing of the novel hybrid biosensors and the evaluation of their advantages/disadvantages in terms of monitoring DNA damage caused by an UV light irradiation employing various voltammetric methods (square wave (SWV), linear sweep (LSV), or cyclic (CV) voltammetry) and elec-trochemical impedance spectroscopy (EIS) is also presented.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/GC20-01417J" target="_blank" >GC20-01417J: Nové přístupy zabraňující pasivaci elektrod při monitorování environmentálních organických polutantů</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Electroanalytical Chemistry
ISSN
1572-6657
e-ISSN
1873-2569
Svazek periodika
908
Číslo periodika v rámci svazku
March
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
9
Strana od-do
116095
Kód UT WoS článku
000767857500015
EID výsledku v databázi Scopus
2-s2.0-85124176855