Novel hybrid electrochemical DNA biosensor for monitoring oxidative DNA damage via oxidation/reduction signals of low molecular weight double-stranded DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10420325" target="_blank" >RIV/00216208:11310/20:10420325 - isvavai.cz</a>

Výsledek na webu

<a href="http://web.natur.cuni.cz/analchem/isc/isc16.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc16.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Novel hybrid electrochemical DNA biosensor for monitoring oxidative DNA damage via oxidation/reduction signals of low molecular weight double-stranded DNA

Popis výsledku v původním jazyce

Deoxyribonucleic acid (DNA) represents a major target molecule for many damaging agents causing unfavorable changes in a structure of DNA molecule that bind and interact with DNA. Thus, a high demand for reliable tools regarding a better comprehension of the nature of DNA damaging processes still represents one of the main goals in this area. Herein, we describe a development of a novel hybrid electrochemical DNA biosensor based on an &quot;edge-plane&quot; pyrolytic graphite electrode (EPPGE) in connection with an elementary optimization process providing a closer resolution of the redox processes of low molecular weight double-stranded DNA (dsDNA) at the EPPGE. Subsequent analytical application incorporating an employment of the model structure K [IrCl ] (representative 2 6 of transition metal complexes), and evaluation of its damaging effect in relation to DNA by means of linear sweep voltammetry resp. square-wave voltammetry are also presented.

Název v anglickém jazyce

Novel hybrid electrochemical DNA biosensor for monitoring oxidative DNA damage via oxidation/reduction signals of low molecular weight double-stranded DNA

Popis výsledku anglicky

Deoxyribonucleic acid (DNA) represents a major target molecule for many damaging agents causing unfavorable changes in a structure of DNA molecule that bind and interact with DNA. Thus, a high demand for reliable tools regarding a better comprehension of the nature of DNA damaging processes still represents one of the main goals in this area. Herein, we describe a development of a novel hybrid electrochemical DNA biosensor based on an &quot;edge-plane&quot; pyrolytic graphite electrode (EPPGE) in connection with an elementary optimization process providing a closer resolution of the redox processes of low molecular weight double-stranded DNA (dsDNA) at the EPPGE. Subsequent analytical application incorporating an employment of the model structure K [IrCl ] (representative 2 6 of transition metal complexes), and evaluation of its damaging effect in relation to DNA by means of linear sweep voltammetry resp. square-wave voltammetry are also presented.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 16th International Students Conference &quot;Modern Analytical Chemistry&quot;

ISBN

978-80-7444-079-3

ISSN

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e-ISSN

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Počet stran výsledku

7

Strana od-do

83-89

Název nakladatele

Univerzita Karlova, Přírodovědecká fakulta

Místo vydání

Praha

Místo konání akce

Prague

Datum konání akce

17. 9. 2020

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000623101400015