Spectrophotometric determination of ligand-protein ratio in B2M3-DTPA monoclonal conjugates based on yttrium-methylthymol blue complex

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10381619" target="_blank" >RIV/00216208:11310/18:10381619 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s00706-018-2248-1" target="_blank" >https://doi.org/10.1007/s00706-018-2248-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-018-2248-1" target="_blank" >10.1007/s00706-018-2248-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Spectrophotometric determination of ligand-protein ratio in B2M3-DTPA monoclonal conjugates based on yttrium-methylthymol blue complex

Popis výsledku v původním jazyce

Monoclonal conjugates using radionuclides are widely used in the diagnosis or therapy of cancer. A number of ligand molecules per protein molecule (L/P ratio) are a crucial factor affecting the monoclonal conjugate efficacy and safety. For proper and accurate dosing of monoclonal conjugates, it is necessary to establish the L/P ratio before they are applied. The aim of this work was the development of a simple, efficient, non-radioactive, and low-cost spectrophotometric method for L/P ratio measurement. The method is based on transchelation reaction between the monoclonal conjugate and yttrium(III)-methylthymol blue complex followed at wavelength of 590 nm. The method operates in the L/P ratio range of 0.32-8.00 and the results are satisfactorily comparable to the conventional mass spectrometric method.

Název v anglickém jazyce

Spectrophotometric determination of ligand-protein ratio in B2M3-DTPA monoclonal conjugates based on yttrium-methylthymol blue complex

Popis výsledku anglicky

Monoclonal conjugates using radionuclides are widely used in the diagnosis or therapy of cancer. A number of ligand molecules per protein molecule (L/P ratio) are a crucial factor affecting the monoclonal conjugate efficacy and safety. For proper and accurate dosing of monoclonal conjugates, it is necessary to establish the L/P ratio before they are applied. The aim of this work was the development of a simple, efficient, non-radioactive, and low-cost spectrophotometric method for L/P ratio measurement. The method is based on transchelation reaction between the monoclonal conjugate and yttrium(III)-methylthymol blue complex followed at wavelength of 590 nm. The method operates in the L/P ratio range of 0.32-8.00 and the results are satisfactorily comparable to the conventional mass spectrometric method.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie - Chemical Monthly

ISSN

0026-9247

e-ISSN

—

Svazek periodika

149

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

5

Strana od-do

1653-1657

Kód UT WoS článku

000442501400022

EID výsledku v databázi Scopus

2-s2.0-85051640199