Development of miniaturized extraction method used for GC-NCD screening of non-volatile nitroso compounds in malt

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10409571" target="_blank" >RIV/00216208:11310/18:10409571 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60193697:_____/18:N0000068

Výsledek na webu

<a href="https://web.natur.cuni.cz/analchem/isc-mac/" target="_blank" >https://web.natur.cuni.cz/analchem/isc-mac/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of miniaturized extraction method used for GC-NCD screening of non-volatile nitroso compounds in malt

Popis výsledku v původním jazyce

The aim of this study was to develop a miniaturized extraction method for a fast screening of non-volatile nitroso compounds using gas chromatography with a nitroso specific chemiluminescence detection. According to a final methodology, the samples were prepared by extraction of grinded malt using a mixture of pyridine and acetonitrile in ratio 60:40 (v/v). To enhance volatility of the determined analytes, the two-step derivatization using hexamethyldisalazane and N,O-bis(trimethylsilyl)trifluoroacetamide was used. The total volume of the sample was 200 mu l and the preparation time after optimization was 80 min. The extraction method was connected to a classification method, which can divide chromatographic peaks into the groups of N-nitroso and C-nitroso compounds, and interfering substances. After application of the methods mentioned above to real malt samples, the specific chromatographic peaks of C-nitroso and N-nitroso compounds were selected.

Název v anglickém jazyce

Development of miniaturized extraction method used for GC-NCD screening of non-volatile nitroso compounds in malt

Popis výsledku anglicky

The aim of this study was to develop a miniaturized extraction method for a fast screening of non-volatile nitroso compounds using gas chromatography with a nitroso specific chemiluminescence detection. According to a final methodology, the samples were prepared by extraction of grinded malt using a mixture of pyridine and acetonitrile in ratio 60:40 (v/v). To enhance volatility of the determined analytes, the two-step derivatization using hexamethyldisalazane and N,O-bis(trimethylsilyl)trifluoroacetamide was used. The total volume of the sample was 200 mu l and the preparation time after optimization was 80 min. The extraction method was connected to a classification method, which can divide chromatographic peaks into the groups of N-nitroso and C-nitroso compounds, and interfering substances. After application of the methods mentioned above to real malt samples, the specific chromatographic peaks of C-nitroso and N-nitroso compounds were selected.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 14th International Students Conference “Modern Analytical Chemistry“

ISBN

978-80-7444-059-5

ISSN

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e-ISSN

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Počet stran výsledku

9

Strana od-do

105-113

Název nakladatele

Univerzita Karlova, Přírodovědecká fakulta

Místo vydání

Praha 2

Místo konání akce

Prague

Datum konání akce

20. 9. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000495572500018