Development of miniaturized extraction method used for GC-NCD screening of non-volatile nitroso compounds in malt

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F18%3AN0000068" target="_blank" >RIV/60193697:_____/18:N0000068 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/18:10409571

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of miniaturized extraction method used for GC-NCD screening of non-volatile nitroso compounds in malt

Popis výsledku v původním jazyce

The aim of this study was to develop a miniaturized extraction method for a fast screening of non-volatile nitroso compounds using gas chromatography with a nitroso specific chemiluminescence detection. According to a final methodology, the samples were prepared by extraction of grinded malt using a mixture of pyridine and acetonitrile in ratio 60:40 (v/v). To enhance volatility of the determined analytes, the two-step derivatization using hexamethyldisalazane and N,O bis(trimethylsilyl)trifluoroacetamide was used. The total volume of the sample was 0,2 ml and the preparation time after optimization was 80 min. The extraction method was connected to a classification method, which can divide chromatographic peaks into the groups of N-nitroso and C-nitroso compounds, and interfering substances. After application of the methods mentioned above to real malt samples, the specific chromatographic peaks of C-nitroso and N-nitroso compounds were selected.

Název v anglickém jazyce

Development of miniaturized extraction method used for GC-NCD screening of non-volatile nitroso compounds in malt

Popis výsledku anglicky

The aim of this study was to develop a miniaturized extraction method for a fast screening of non-volatile nitroso compounds using gas chromatography with a nitroso specific chemiluminescence detection. According to a final methodology, the samples were prepared by extraction of grinded malt using a mixture of pyridine and acetonitrile in ratio 60:40 (v/v). To enhance volatility of the determined analytes, the two-step derivatization using hexamethyldisalazane and N,O bis(trimethylsilyl)trifluoroacetamide was used. The total volume of the sample was 0,2 ml and the preparation time after optimization was 80 min. The extraction method was connected to a classification method, which can divide chromatographic peaks into the groups of N-nitroso and C-nitroso compounds, and interfering substances. After application of the methods mentioned above to real malt samples, the specific chromatographic peaks of C-nitroso and N-nitroso compounds were selected.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LO1312" target="_blank" >LO1312: Výzkumné senzorické centrum v Praze a Výzkumná a vývojová varna - udržitelnost a rozvoj</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 14th International Students Conference

ISBN

978-80-7444-059-5

ISSN

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e-ISSN

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Počet stran výsledku

9

Strana od-do

105-113

Název nakladatele

Charles University, Faculty of Science

Místo vydání

Prague

Místo konání akce

Prague

Datum konání akce

20. 9. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000495572500018