Inkjet-Printed Carbon Nanotube Electrodes for Measuring Pyocyanin and Uric Acid in a Wound Fluid Simulant and Culture Media

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10396642" target="_blank" >RIV/00216208:11310/19:10396642 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=qzae8MqqmE" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=qzae8MqqmE</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.analchem.8b05591" target="_blank" >10.1021/acs.analchem.8b05591</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Inkjet-Printed Carbon Nanotube Electrodes for Measuring Pyocyanin and Uric Acid in a Wound Fluid Simulant and Culture Media

Popis výsledku v původním jazyce

Polyacrylamide-coated, carbon nanotube (PA/CNT) electrodes were prepared by an inkjet printing process and used to measure pyocyanin and uric acid in a wound fluid simulant at 37 degrees C. These two molecules are potential indicators of infection, and therefore their detection could prove useful for monitoring wound healing. Pyocyanin is a marker for the common wound bacterium Pseudomonas aeruginosa. Our long-term goal is to use these inexpensive and disposable electrodes to measure biomarkers of wound healing directly. In this proof-of-concept work, studies were performed in a wound fluid simulant to evaluate the stability of the electrodes and their responsiveness for the two bioanalytes. The PA/CNT inkjet-printed electrodes and electrical contacts were stable with unchanging physical and electrochemical properties in the wound fluid simulant over a 7-8-day period at 37 degrees C. The detection figures of merit for pyocyanin in the simulant at 37 degrees C were as follows: linear over the physiologically relevant range = 0.10 to 100 mu mol L-1 (R-2 = 0.9992), limit of detection = 0.10 mu mol L-1 (S/N = 3), sensitivity = 35.6 +/- 0.8 mA-L mol(-1) and response variability &lt;= 4% RSD. The detection figures of merit for uric acid in the simulant at 37 degrees C were as follows: linear over the physiologically relevant range = 100 to 1000 mu mol L-1 (R-2 = 0.9997), sensitivity = 2.83 +/- 0.01 mA-L mol(-1), and response variability &lt;= 4% RSD. The limit of detection was not determined. The PA/CNT electrodes were also used to quantify pyocyanin concentrations in cell-free culture media from different strains of P. aeruginosa. The detected concentrations ranged from 1.00 +/- 0.02 to 118 +/- 6 mu M depending on the strain.

Název v anglickém jazyce

Inkjet-Printed Carbon Nanotube Electrodes for Measuring Pyocyanin and Uric Acid in a Wound Fluid Simulant and Culture Media

Popis výsledku anglicky

Polyacrylamide-coated, carbon nanotube (PA/CNT) electrodes were prepared by an inkjet printing process and used to measure pyocyanin and uric acid in a wound fluid simulant at 37 degrees C. These two molecules are potential indicators of infection, and therefore their detection could prove useful for monitoring wound healing. Pyocyanin is a marker for the common wound bacterium Pseudomonas aeruginosa. Our long-term goal is to use these inexpensive and disposable electrodes to measure biomarkers of wound healing directly. In this proof-of-concept work, studies were performed in a wound fluid simulant to evaluate the stability of the electrodes and their responsiveness for the two bioanalytes. The PA/CNT inkjet-printed electrodes and electrical contacts were stable with unchanging physical and electrochemical properties in the wound fluid simulant over a 7-8-day period at 37 degrees C. The detection figures of merit for pyocyanin in the simulant at 37 degrees C were as follows: linear over the physiologically relevant range = 0.10 to 100 mu mol L-1 (R-2 = 0.9992), limit of detection = 0.10 mu mol L-1 (S/N = 3), sensitivity = 35.6 +/- 0.8 mA-L mol(-1) and response variability &lt;= 4% RSD. The detection figures of merit for uric acid in the simulant at 37 degrees C were as follows: linear over the physiologically relevant range = 100 to 1000 mu mol L-1 (R-2 = 0.9997), sensitivity = 2.83 +/- 0.01 mA-L mol(-1), and response variability &lt;= 4% RSD. The limit of detection was not determined. The PA/CNT electrodes were also used to quantify pyocyanin concentrations in cell-free culture media from different strains of P. aeruginosa. The detected concentrations ranged from 1.00 +/- 0.02 to 118 +/- 6 mu M depending on the strain.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Centrum nových přístupů k bioanalýze a molekulární diagnostice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Chemistry

ISSN

0003-2700

e-ISSN

—

Svazek periodika

91

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

8835-8844

Kód UT WoS článku

000476567700019

EID výsledku v databázi Scopus

2-s2.0-85069949132