Determination of short-chain fatty acids in feces by capillary electrophoresis with indirect UV-VIS detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10399165" target="_blank" >RIV/00216208:11310/19:10399165 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=hTVOc7CvZy" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=hTVOc7CvZy</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c9ay01202h" target="_blank" >10.1039/c9ay01202h</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of short-chain fatty acids in feces by capillary electrophoresis with indirect UV-VIS detection

Popis výsledku v původním jazyce

Fermentation of undigested carbohydrates and amino acids in the large intestine leads to the production of short-chain fatty acids (SCFAs). Increasing attention has been recently paid to investigating the effect of the presence of SCFAs in the mammalian large intestine on the colon health. Thus, clinical practice requires analytical methods capable of fast, reliable and inexpensive quantification of SCFAs. Capillary electrophoresis meets such requirements, and hence a capillary electrophoresis method was developed to quantify acetate, propionate, butyrate and isobutyrate in human and canine feces. Benzoic acid in the background electrolyte was selected as a suitable indirect probe for indirect UV-VIS detection. A considerable advantage of the proposed method is the determination of butyric acid as well as isobutyric acid in one analytical run. The separation of these isomers was achieved through addition of alpha-cyclodextrin to the background electrolyte. The optimized method offers quantification of acetate at concentrations above 10 mu M, and propionate, butyrate and isobutyrate at concentrations above 8 mu M with acceptable precision, accuracy and with no significant matrix effects.

Název v anglickém jazyce

Determination of short-chain fatty acids in feces by capillary electrophoresis with indirect UV-VIS detection

Popis výsledku anglicky

Fermentation of undigested carbohydrates and amino acids in the large intestine leads to the production of short-chain fatty acids (SCFAs). Increasing attention has been recently paid to investigating the effect of the presence of SCFAs in the mammalian large intestine on the colon health. Thus, clinical practice requires analytical methods capable of fast, reliable and inexpensive quantification of SCFAs. Capillary electrophoresis meets such requirements, and hence a capillary electrophoresis method was developed to quantify acetate, propionate, butyrate and isobutyrate in human and canine feces. Benzoic acid in the background electrolyte was selected as a suitable indirect probe for indirect UV-VIS detection. A considerable advantage of the proposed method is the determination of butyric acid as well as isobutyric acid in one analytical run. The separation of these isomers was achieved through addition of alpha-cyclodextrin to the background electrolyte. The optimized method offers quantification of acetate at concentrations above 10 mu M, and propionate, butyrate and isobutyrate at concentrations above 8 mu M with acceptable precision, accuracy and with no significant matrix effects.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Methods

ISSN

1759-9660

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

36

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

4575-4579

Kód UT WoS článku

000487048000003

EID výsledku v databázi Scopus

2-s2.0-85072626461