Mastné kyseliny ovlivňují virulenci Salmonella Typhimurium a snižují její schopnost kolonizovat střevní trakt prasat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F08%3A%230000416" target="_blank" >RIV/00027162:_____/08:#0000416 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Coated fatty acids alter virulence properties of Salmonella Typhimurium and decrease intestinal colonization of pigs

Popis výsledku v původním jazyce

The aim of the present study was to determine whether short- (SCFA) and medium-chain fatty acids (MCFA) are able to alter virulence gene expression and to decrease Salmonella Typhimurium colonization and shedding in pigs. Minimal inhibitory concentrations (MIC) of 4 SCFA (formic acid, acetic acid, propionic acid, butyric acid) and 2 MCFA (caproic and caprylic acid) were determined using 54 porcine Salmonella Typhimurium field strains. Expression of hilA and invasion in porcine intestinal epithelial cells was significantly lower when bacteria were grown in LB-broth containing sub-MIC concentrations of butyrate or propionate (10mM) and caproic or caprylic acid (2mM). When given as feed supplement to pigs experimentally infected with Salmonella Typhimurium, coated butyric acid was shown to be effective in decreasing the levels of faecal shedding and intestinal colonization. Uncoated fatty acids, however, did not influence fecal shedding, intestinal or tonsillar colonization in pigs.

Název v anglickém jazyce

Coated fatty acids alter virulence properties of Salmonella Typhimurium and decrease intestinal colonization of pigs

Popis výsledku anglicky

The aim of the present study was to determine whether short- (SCFA) and medium-chain fatty acids (MCFA) are able to alter virulence gene expression and to decrease Salmonella Typhimurium colonization and shedding in pigs. Minimal inhibitory concentrations (MIC) of 4 SCFA (formic acid, acetic acid, propionic acid, butyric acid) and 2 MCFA (caproic and caprylic acid) were determined using 54 porcine Salmonella Typhimurium field strains. Expression of hilA and invasion in porcine intestinal epithelial cells was significantly lower when bacteria were grown in LB-broth containing sub-MIC concentrations of butyrate or propionate (10mM) and caproic or caprylic acid (2mM). When given as feed supplement to pigs experimentally infected with Salmonella Typhimurium, coated butyric acid was shown to be effective in decreasing the levels of faecal shedding and intestinal colonization. Uncoated fatty acids, however, did not influence fecal shedding, intestinal or tonsillar colonization in pigs.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Microbiology

ISSN

0378-1135

e-ISSN

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Svazek periodika

132

Číslo periodika v rámci svazku

3-4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000260973900010

EID výsledku v databázi Scopus

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