The interconnection between cytokeratin and cell membrane-bound beta-catenin in Sertoli cells derived from juvenile Xenopus tropicalis testes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10409396" target="_blank" >RIV/00216208:11310/19:10409396 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=X.vClEdFe6" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=X.vClEdFe6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/bio.043950" target="_blank" >10.1242/bio.043950</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The interconnection between cytokeratin and cell membrane-bound beta-catenin in Sertoli cells derived from juvenile Xenopus tropicalis testes

Popis výsledku v původním jazyce

Sertoli cells (SCs) play a central role in the determination of male sex during embryogenesis and spermatogenesis in adulthood. Failure in SC development is responsible for male sterility and testicular cancer. Before the onset of puberty, SCs are immature and differ considerably from mature cells in post-pubertal individuals regarding their morphology and biochemical activity. The major intermediate filament (IF) in mature SCs is vimentin, anchoring germ cells to the seminiferous epithelium. The collapse of vimentin has resulted in the disintegration of seminiferous epithelium and subsequent germ cell apoptosis. However, another IF, cytokeratin (CK) is observed only transiently in immature SCs in many species. Nevertheless, its function in SC differentiation is poorly understood. We examined the interconnection between CK and cell junctions using membrane beta-catenin as a marker during testicular development in the Xenopus tropicalis model. Immunohistochemistry on juvenile (5 months old) testes revealed co-expression of CK, membrane beta-catenin and E-cadherin. Adult (3-year-old males) samples confirmed only E-cadherin expression; CK and beta-catenin were lost. To study the interconnection between CK and beta-catenin-based cell junctions, the culture of immature SCs (here called XtiSCs) was employed. Suppression of CK by acrylamide in XtiSCs led to breakdown of membrane-bound beta-catenin but not F-actin and beta-tubulin or cell-adhesion proteins (focal adhesion kinase and integrin (31). In contrast to the obvious dependence of membrane beta-catenin on CK stability, the detachment of beta-catenin from the plasma membrane via uncoupling of cadherins by Ca2+ chelator EGTA had no effect on CK integrity. Interestingly, CHIR99021, a GSK3 inhibitor, also suppressed the CK network, resulting in the inhibition of XtiSCs cell-to-cell contacts and testicular development in juvenile frogs. This study suggests a novel role of CK in the retention of beta-catenin-based junctions in immature SCs, and thus provides structural support for seminiferous tubule formation and germ cell development.

Název v anglickém jazyce

The interconnection between cytokeratin and cell membrane-bound beta-catenin in Sertoli cells derived from juvenile Xenopus tropicalis testes

Popis výsledku anglicky

Sertoli cells (SCs) play a central role in the determination of male sex during embryogenesis and spermatogenesis in adulthood. Failure in SC development is responsible for male sterility and testicular cancer. Before the onset of puberty, SCs are immature and differ considerably from mature cells in post-pubertal individuals regarding their morphology and biochemical activity. The major intermediate filament (IF) in mature SCs is vimentin, anchoring germ cells to the seminiferous epithelium. The collapse of vimentin has resulted in the disintegration of seminiferous epithelium and subsequent germ cell apoptosis. However, another IF, cytokeratin (CK) is observed only transiently in immature SCs in many species. Nevertheless, its function in SC differentiation is poorly understood. We examined the interconnection between CK and cell junctions using membrane beta-catenin as a marker during testicular development in the Xenopus tropicalis model. Immunohistochemistry on juvenile (5 months old) testes revealed co-expression of CK, membrane beta-catenin and E-cadherin. Adult (3-year-old males) samples confirmed only E-cadherin expression; CK and beta-catenin were lost. To study the interconnection between CK and beta-catenin-based cell junctions, the culture of immature SCs (here called XtiSCs) was employed. Suppression of CK by acrylamide in XtiSCs led to breakdown of membrane-bound beta-catenin but not F-actin and beta-tubulin or cell-adhesion proteins (focal adhesion kinase and integrin (31). In contrast to the obvious dependence of membrane beta-catenin on CK stability, the detachment of beta-catenin from the plasma membrane via uncoupling of cadherins by Ca2+ chelator EGTA had no effect on CK integrity. Interestingly, CHIR99021, a GSK3 inhibitor, also suppressed the CK network, resulting in the inhibition of XtiSCs cell-to-cell contacts and testicular development in juvenile frogs. This study suggests a novel role of CK in the retention of beta-catenin-based junctions in immature SCs, and thus provides structural support for seminiferous tubule formation and germ cell development.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology Open [online]

ISSN

2046-6390

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

bio043950

Kód UT WoS článku

000506171400005

EID výsledku v databázi Scopus

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